Each lot is carefully controlled to ensure the absence of non-specific endonucleases, as well as 3' 5' exonuclease, DNA nicking, RNase and for first strand cDNA synthesis activities. Lot to lot reproducibility is guaranteed.

Store at -20ºC (except gel form, at 4ºC).

Users may be required to obtain a license depending on the country and/or application.

UptiReverse cDNA/DNA Polymerase kit is the system of choice for reverse transcribing RNA to cDNA and amplifying this cDNA, in one step. As reverse transcription is performed at high temperatures(65 ºC or even at 70 ºC), secondary structures present in RNA are avoided, thus preventing inefficient or in complete transcription that may appear using viral reverse transcriptases at low temperatures. Performance of the whole process in one single tube dramatically lowers risk of contaminations, and total reaction time is around 2 hours.

UptiReverse cDNA/DNA Polymerase can accept labeled nucleotides as substrates (radiolabeled, digoxigenin, biotin, fluorescent nucleotides). The efficiency of reverse transcription ensures amplification of RNA up to 1 Kbp. The kit can be used either total RNA (10 pg - 1 µg) or mRNA (1 pg - 100 ng), using specific primers, or random primers or oligodT with mRNA (alternative protocol).

The kit contains all necessary reagents for performance of RT-PCR: reverse transcription buffer (RNA-buffer), amplification buffer (DNA-buffer), dNTP and UptiReverse cDNA/DNA Polymerase. For MgCl2 optimization in the amplification reaction, kits with DNA-buffer without Mg are available.

As shown in the picture, the UptiReverse cDNA/DNA polymerase is able to detect specific fragments using down to 1 pg in 35 cycles of amplification, with a high specificity.