IMMUNIZATION

Protein Carriers

• its own ability to stimulate the immune response

• its ability to be adequately coupled to the desired hapten

• KLH is a classic and efficient used carrier for small haptens

• Bovine serum albumin and Ovalbumin are good carriers too

• MaxiBind BSA OVA bLG are improved carriers for creating multivalent antigens

BSA Highest Purity

UP909382

10g

Powder

Suits Molecular Biology for the most exigent applications, notably as stabilizing of immuno or genomic reagents

Purity >99%

DNAase, RNase, Ig Free

pH5-5.4(% in water)

Cold ethanol fractionnation

US origin

Ovalbumin, from Hen Eggs

Powder

UPR5851B

UPR5851A

1g

5g

KLH, in PBS

UP885021

UP885022

100 mg

5x100 mg

KLH, in BES

UP835990

UP865991

100 mg

5x100 mg

The major protein of egg white

CAS [9006-59-1]

• Glycoprotein, MW 45,000 Da

• Purity > 98%

• Soluble up 50 mg/ml in water

Ovalbumin is used as carrier protein to create immunoconjugates with peptides. Beside excellent immunogenicity, conjugates are also used for immunoassays, notably to discriminate anti carrier antibodies.

Ovalbumin may also be used as control protein (molecular weight marker, irrelevant protein…), for saturation of coatings, and for research purposes (protein interactions…).

Keyhole Limpet Hemocyanin from Megathura crenulata

• 90% of non-dissociated hemocyanine

• Coper/protein Ratio: 0.00249

• Contains a proprietary preservative agent

• Purity (exclusion chromatography): > 90%

• Solubility: 5-10 mg/ml in saline buffer

KLH stimulates very efficiently the immune response compared to other carriers, thanks to its big size.

Uptima KLH offers enhanced purity and solubility.

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Tel (33) (0) 4 70 03 88 55 -  Fax (33) (0) 4 70 03 82 60 - uptima@interchim.com  www.interchim.com