Molecular Biology

Competent Cells

 

 

 We offer the most popular competent cell strains with exceptional, high efficiency and low cost. For extra protection T1 phage resistance is added without extra cost.

Superior chemically and electrocompetent cells offered in both standard and single-use aliquots.

The cells are EndA- to minimize degradation of plasmid DNA. They contain the LacZ-deltaM15 marker for easy blue/white screening of recombinants. In addition, the GC strains are T1 phage resistant to protect your laboratory from possible T1 infection.

The Competent Cells are available in a wide range of transformation efficiencies:

  • Value Efficiency – 10(8) transformants/µg pUC19

  • High Efficiency – 10(9) transformants/µg pUC19

  • Thunderbolt – 10(10) transformants/µg pUC19

 

If you are using

Our equivalent

Features and Benefits

DH5a

GC5

Chemically competent cells

Same markers/abilities as DH5a

Single-use and standard aliquots

T1 phage resistant

XL1 Blue

JM109 or GC5 (Note: Only JM109 if you are using single-stranded DNA or LacI marker)

Chemically competent cells

Same abilities as XL1-Blue F’

DH10B

GC10

Chemically and electrocompetent cells

Comparable to DH10B Competent Cells

Large plasmids, methylated DNA

Single-use and standard aliquots

T1 phage resistant

 

Competent Cells - Technical Features

T1 Phage and its Relatives

Both GC5 and GC10 Competent Cells protect you work and laboratory from infection by bacteriophage T1 or one of its ugly relatives.

Why is the T1 resistance marker nice to have?

  • T1 and its relatives are nasty phages that kill E.coli.

  • T1 is present in some libraries of cDNA clones that get passed around between labs and genome centers.

  • If you have experienced T1 phage contamination, you will never forget it. It’s total devastation for your clones.

  • The T1 resistance marker protects your clones. It is present at no additional cost.

Blue / White Screening

Blue/White screening is a popular tool for quick and easy identification of recombinants.

The GC5, GC10 and JM109 strains all carry the LacZ-deltaM15 marker that enables blue/white screening.

 

Product

Catalog Number

Quantity

GC5 High Efficiency >10(9) cfu, Single Use Aliquots

UPAM889B

10x50µl

GC5 High Efficiency >10(9) cfu, Single Use Aliquots

UPAM889A

20x50µl

GC5 High Efficiency >10(9) cfu, Standard Aliquots

UPAM889C

5x200µl

GC5 Value Efficiency >10(8) cfu

UPAM893A

10x200µl

GC10 High Efficiency >10(9) cfu, Single Use Aliquots

UPAL709C

10x50µl

GC10 High Efficiency >10(9) cfu, Single Use Aliquots

UPAL709B

20x50µl

GC10 High Efficiency >10(9) cfu, Standard Aliquots

UPAL709A

5x200µl

ElectroCompetent cells

Thunderbolt GC10 ElectroCompetent >10(10) cfu

UPAM885A

5x80µl

Thunderbolt GC10 ElectroCompetent >10(10) cfu

UPAM885B

75x80µl

Thunderbolt GC10 ElectroCompetent >10(10) cfu

UPAM885C

5x100µl

Thunderbolt GC10 ElectroCompetent >10(10) cfu

UPAM885D

75x100µl

SOC Medium

UPAN146A

10x 10ml

 

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