Molecular Biology

Electrophoresis buffer

 

 

 

TAE buffer

UP892574

1.6L

TBE buffer

UP86510A

5L

(Tris-Acetate-EDTA), 25X Concentrate

Molecular Biology Grade

(Tris-Borate-EDTA), 10X Concentrate

Molecular Biology Grade

 UP86510C

4x5L

Better resolution of fragments greater than 4 kb

A pH adjusted solution of Ultra Pure Grade components, which when reconstituted to a working concentration, contains 0.040M Tris and 0.001M EDTA.

Applications:

DNA electrophoresis on agarose gel in native conditions

pH (1X concentration) @ 25°C 8.2-8.4

UltraPure Components

Protease, DNAse, RNAse free

Microfiltered

10X liquid concentrate can be used to easily prepare a 1X working solution by diluting with distilled, deionized water.

A single strenght (1X) solution contains 0.089 M Tris Base, 0,089 M Borate and 0,002 M EDTA.

pH (1 :10) @ 25°C : 8.2-8.4

UltraPure Components

Protease, DNAse, RNAse free

Microfiltered

 

TBE buffer is commonly used in all DNA electrophoresis applications (both for acrylamide and for agarose gels), including sequencing. In general, TBE buffer offers better resolution of 0.1 to 3 kb fragments; TBE is better suited for high-voltage (>150V) electrophoresis because of its higher buffering capacity and lower conductivity than TAE.

TG-SDS buffer

UP914957

4L

(Tris-Glycine-Sodium Dodecyl Sulfate), 10X Concentrate

Molecular Biology Grade

A concentrated (10X), pH adjusted solution of Ultra Pure Tris Buffer, Biotech Glycine & Sodium Dodecyl Sulfate. There is a sufficient concen_çà)=trate to prepare 40 l of 1X TG-SDS Buffer.

pH (1X, Water) @25°C : 8.30-8.70

UltraPure Components

Protease, DNAse, RNAse free

 

 

Related products

SSC buffer

UP586046

4L

High qality buffer for southern and northern.

Each liter when diluted with distilled desionized water gives a 1Xsolution of NaCl 0.15M, sodium citrate 0.015M

Blotting Reagents - See Immunodetection

PBS

TBS

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