Cell Biology Tools

cell culture reagents

Hybridokine

 

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Hybridokine

UP826430

12,5ml

 

Hybridoma and growth factors

Myeloma and hybridoma are tumoral cell lines that have the ability to proliferate indefinitely. However most require growth factors to maintain optimal growth. One important application is the production of monoclonal antibodies that are recovered in the cell culture medium.

On key step in the hybridoma obtention is the cloning. That consists to obtain a culture from a unique cell. This critical step should be done rapidly after parent cells where fused. It is recommanded to repeat cloning from time to time to avoid the developpment of non interesting cells that may appear and overcome the growth of the right cell line (producing the desired specific antibody).

Many works tried to improve cell culture media and supplements.

  • To promote the optimal growth of hybridoma, cell culture media should be supplemented with cytokines and Foetal Calf Serum (FCS). However added proteins often interfere with further treatment, like antibody purification.

  • Macrophages feeders are frequently used in hybridoma cloning, but their preparation is a hassle and gives tedious or variable results.

Now, Hybridokine is a fantastic breakthrought for hybridoma culture!

Cloning yield up 80%

Efficient for the stabilization of hybridoma of

  • mouse / mouse (X63, SP2/0, NS1)

  • rat / rat (Y3)

  • mouse / human

  • mouse / rat

Helps the culture of cryo-protected cells

The ‘Hybridokine’ (Hybridoma cloning enhancing factor) is a growth factor that is obtained in culture media for continuous culture of a human tumoral cell line (ES1, a strain of Ewing sarcoma). It contains a potent growth factor that allows and accelerates the growth of cells, even unique cell. It is a mixture whose main activity relay on an undefined cytokine, and Interleukine 6.

Note : The Hybridokine is not used in place of serum, thus the cloning medium should be supplemented with FCS.

Hybridokine is offered sterile and lyophilized, stable 2 years. When reconstitued with water, the stability is 6 months at +4°C.

Hybridokine is thus an efficient and economical tool for the cloning of many strains: mouse / mouse (X63, SP2/0, NS1); rat / rat (Y3); mouse / human ; mouse / rat; Hybridoma obtained from other species were not assayed at this time. It helps too cells to grow again after a cryo-storage, and for in vitro immunizations.

Technical Sheet

Literature

1- De Leij, L., Schwander, E. and T.H. The. Cryopreservation in hybridoma production. In : methods of hybridoma formation. Eds. Bartal, A., and Hirshaut, Y., The Humana Press, Clifton, New Jersey, p 419-427, (1987).

2- Boldicke, T., Kindt, S., Mayvald, F., Fitzlaff, G., Bocher, M., Frank, R., and J. Collins. Production of specific monoclonal antibodies against the active sites of human pancreatic secretory trypsin inhibitor by in vitro immunization with synthetic peptides. Eur. J. Biochem. 175, 259-264 (1988).

 

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