Immunodetection

(Strept)avidin reagents

 

(Strept)avidin conjugates from Uptima are high quality reagents to be used in various immunodetections (ELISA, Blotting, Cytometry, Immunohistology).

 

Streptavidin is isolated from Streptomyces avidinii, and has a very high affinity for biotin (>10-14 M-1). This makes the -biotin interaction an ideal tool for many research applications. (Strept)avidin does not have any carbohydrates and has a lower ionic charge than avidin, resulting in a lower non-specific binding. This makes (strept)avidin a preferred choice for many biotin-based applications.

Avidin is purified from eggs and has an even higher affinity for biotin (>10-15 M-1 ). It lack of the RDY sequence found in Streptavidin, that is homologous of some integrins, giving the advantage of giving no unspecific binding that is observed in some detection systems with Streptavidin. Avidin presents however glycones and a higher ionic net charge, that generated in some application higher background. Uptima offers to that point a chemically modified avidin, gathering the advantage of high specificity and affinity of avidin, but lowest background of Streptavidin. This neutralized avidin gives unsurpassed performences.

Both (Strept)avidin and NeutralizedAvidin are offered unlabeled, for coating, and labeled by enzymes and fluorophores for detections in ELISA, blotting, FCM, and IH techniques.

Technical Sheet

  • Labeled (Strept)avidin offers several advantages, compared with directly labeled primary antibodies :

    • Lower background

    • Amplified detection signals

    • Easier to calibrate than different I or II antibodies

    • More convenient for rarely used antibodies

    • Increased flexibility

Ask Uptima for other labels

(PhycoErythrine, Texas Red…)

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Unlabeled

FITC

Peroxidase

Alkaline Phosphatase

(Strept)avidin

UP51558C

UP277130

UP395880

UP518490

-

5mg

1 mg

1mg

1mg

-

UP51558B

-

-

-

-

2mg

-

-

-

Neutralized Avidin

UP25527A

UP73578A

UP36570A

UP38592A

NEW

5mg

1 mg

1mg

1mg

Avidin

UP39860D

Inquire

Inquire

Inquire

-

5mg

-

-

-

 

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Tel (33) (0) 4 70 03 88 55 -  Fax (33) (0) 4 70 03 82 60 - uptima@interchim.com  www.interchim.com