Immunodetection

BUFFERS AND SATURATING AGENTS

Albumins - Normal Sera and IgG

Albumins

• BSA Biotech Grade (Fraction V)

• BSA Immuno-hemato Grade (Fraction V)

• BSA Powders

• Maxibind BSA

Normal Sera and IgG

• Technical Information

• Applications

• Normal Sera and Immunoglobulins

• Related Products

Albumins

Albumin is commonly used as an efficient blocking agent and diluting buffers for immunoassays to reduce background caused by non-specific binding, and therefore to increase assay sensitivity.

See also other saturating agents

BSA Biotech Grade 

UP900100

50 ml

BSA Immuno-hemato Grade

UP900130

50 ml

(Fraction V)

500 ml

(Fraction V)

Suitable for most immunotechnologies (saturation after coating, in Elisa, IHC and blots, to dilute specific immuno-reagents (ELISA, blot, CMF)...

Suitable for agglutination and immunohistology

Contains 7,8g/L NaCl.

BSA Powders

BSA standard grade

BSA low salt

UP32890

Maxibind BSA

UP84225

A cationised BSA for special coating/conjugations

Normal Sera and IgG

These reagents are prepared from blood of healthy animals. They are typically used as controls or as additives in various immuno-techniques.

Store at -20°C, shelf life of 2 years.

Applications

Normal Serum

The serum is collected after blood coagulation. After addition of 0.09% of sodium azide as preservative, it is filtered using a 0.45um filter

Normal IgGAM

Normal IgGAM are whole immunoglobulins purified from normal serum by ammonium precipitation. They consist mainly of IgG (75-85%, mw 180kDa), and IgM (1-5%, mw ~300kDa).

Uptima IgGAM are solubilised at 5mg/ml in PBS buffer with 0.09% sodium azide, and filtered using a 0.45 µm filter.

Inquire for availability.

Normal pure IgG

Immunoglobulins from class G (IgG) have been purified from serum by ammonium and caprylic acid fractionated precipitation to achieve a purity greater than 90% (checked by electrophoresis). IgG concentration is determined by spectrophotometry. Uptima Pure IgG's are solubilised at 5mg/ml in PBS buffer with 0.09% of sodium azide, and filtered using a 0.45um filter. Total quantity is 15 mg (for Bovine, Goat, Human, Rabbit, Swine, Sheep, Stag) or 5 mg (for Mouse, Rat).

• Protein controls and standards for assays or analysis

• Standardized samples (i.e. serum containing 10 µg/ml of a standard hormone)

• Dilution of blood samples (dosage at constant protein concentration…)

• Saturation of tubes, vials, gels, columns, or filters to prevent non-specific adsorbtion of diluted molecules

• Stabilization of reagents

• Saturation of IgG binding sites for immuno-reagents (IgG receptors of cells in FCM, IF and IHC)

• Equilibrium studies (free and bound fractions of a drug in serum)

• Co-precipitation: normal IgGs are added to precipitate a diluted monoclonal

• Living cells : suspension media for cells, opsonisation of bacteria…

• Immunological studies : natural antibodies, auto-immune antibodies, immune complexes…

• Purification of blood components

Normal Sera and Immunoglobulins

-

Normal Serum

Normal Purified IgG

-

Normal Serum

Normal Purified IgG

BOVINE

UP89243A

UP757700

SWINE

UP379021

UP062700

-

10ml

15mg

-

10ml

15mg

HORSE

UP24741A

-

RAT

UP37911A

UP443080

-

10ml

--

-

-

15mg

GOAT

UP379030

UP767090

MOUSE

UP379120

UP386670

-

10ml

15mg

-

2ml

5mg

HUMAN

UP697906

UP408603

CHICKEN

UP37908A

-

-

10ml

15mg

-

10ml

-

RABBIT

UP379060

UP378416

HAMSTER

UP28432A

-

-

10ml

10mg

-

2ml

-

GUINEA-PIG

UP37916

-

DONKEY

UP77719A

-

-

10ml

-

-

10ml

-

SHEEP

UP697927

UP797560

-

-

10ml

15mg

• Like batch reservations

• Bulk quantities

• Plasma

• EDTA

• Citrated

• Or heparined serum; without preservatives…

Related Products

SeaBlock saturating agent

UP40301

Fish serum for saturating in biotechnology

Albumin solution

Albumin Powders

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