Desalting

DIALYSIS / DESALTING

Dialysis with CelluSep Membranes

 

Introduction to CELLUSEP Dialysis Products

Cellu•Sep® Dialysis tubings are the best choice for dialysis, notably in Biology applications, for proteins and nucleic acids. They are regenerated cellulose membranes manufactured with high quality process for optimal and reproducible results

T1, T2, T3 and T4 Cellu•Sep® Dialysis Membranes

suit classical dialysis applications, especially when attention is paid to very low absorption of proteins, low impurity levels , good compatibilities, and economic costs

H1 Cellu•Sep® Dialysis Membranes

suit most exigent applications (minimal contaminants content, ready-to-use individual packages); suit notably DNA desalting

Cellu.Sep membrane closure clamps

Macro Filler funnel - Mini Filler funnel - Silicone loops

Ask for any questions :

  • Sterilisation of membranes

  • Cleaning of heavy metals and sulfides

  • Concentrating sample directly in your dialysis bag!

Cellu•Sep® membranes Performances

Protein absorption

(bovine serum albumin)

<=1mg/cm2

Retention

(T=10 hours, with T2 Membranes)

Cytochrome C (12,3 KDa)

97%

Vitamin B-12 (1355 Da)

59%

Cellu• Sep® T1, T2, T3 and T4 tubular dialysis membranes

Cellu•Sep® T1, T2, T3 et T4 membranes suit most dialysis applications

  • Very low absorption of proteins

  • Pure regenerated cellulose for low impurity levels

  • Nominal Filter ratings : 3500, 6000 and 12000 Daltons

  • Economical

Each package includes :

  • A detailled instruction manual

  • Two clamps

Cellu•Sep® membranes technical data

Cadmium

< 0.1 ppm

Chromium

< 0.1 ppm

Coper

< 0.1 ppm

Iron

13.6 ppm

Nickel 

< 0.1 ppm

Zinc

6.24 - 12.9 ppm

Lead

0.75 - 0.8 ppm

Cellu•Sep® T1, T2, T3 et T4 membranes contain glycerol to prevent brittleness, and are packaged with a dessicant to control humidity.

Technical Sheet


Cellu•Sep® T1 Membranes 

Nominal Filter rating : 3500 Da (4-6000 MWCO)

Flat width

Ø dry 

Vol/cm

Cat. #

Lenght

19 mm 12.1 mm 1.15 T1-19-15 15 m 
46 mm 29.3 mm 6.74 T1-46-15 15 m

Cellu•Sep® T3 Membranes 

Nominal Filter rating : 12000 Da (12-14000 MWCO)

Flat width

Ø dry 

Vol/cm

Cat. #

Lenght

10 mm 6.37 mm 0.32 T3-10-15 15 m
25 mm 15.9 mm 1.98 T3-25-15 15 m
33 mm 21.0 mm 3.46 T3-33-15 15 m
45 mm 28.6 mm 6.42 T3-45-15 15 m
76 mm 48.4 mm 18.4 T3-76-5 5 m

Cellu•Sep® T2 Membranes 

Nominal Filter rating : 6000 Da (6-8000 MWCO)

----------

Flat width

Ø dry 

Vol/cm

Cat.  #

Lenght

10 mm

6.37 mm

0.32

T2-10-15

15 m

23 mm

14.6 mm

1.67

T2-23-15

15 m

25 mm

15.9 mm

1.98

T2-25-15

15 m

32 mm

20.4 mm

3.27

T2-32-15

15 m

40 mm

25.5 mm

5.10

T2-40-15

15 m

50 mm

31.8 mm

7.94

T2-50-15

15 m

Cellu•Sep® T4 Membranes

Nominal Filter rating : 12000 Da (12000 MWCO)

Standard grade (with accessories without technical sheet)

Flat width

Ø dry 

Vol/cm

Cat.  #

Lenght

10 mm

6.37 mm

0.32

T4-10-30 

30 m

25 mm

15.9 mm

1.98

T4-25-30

30 m

33 mm

21.0 mm

3.46

T4-33-30

30 m

45 mm

28.6 mm

6.42

T4-45-30

30 m

 


Cellu•Sep® H1 Membranes

High Grade

suit protein applications, and especially DNA/RNA applications

  • Pre-treated for minimal contaminants content

  • Ready-to-use individual-use packaged

  • MWCO from 1 000 to 25 000 Daltons

Cellu•Sep® H1 are ideal for sensitive applications where glycerol, sulfur compounds, or small amounts of heavy metals will interfere with subsequent steps. Cellu•Sep® H1 membranes are EDTA pretreated and packaged wet in a preservative solution. Individual-use 50cm packages avoid waste, contamination and restorage problems.

Cellu•Sep® H1 provide a efficient tool for purifying DNA and RNA, allowing both dialysis and concentration (ask Uptima).

  • For proteins, notably globular ones, Molecular Weight Cut-Off (MWCO) has become a convenient guide for determining wich membrane to use. Ultimately, however the size and the three-dimensionnal shape of a molecule determine wether it pass or not through a membrane.

  • With the important expansion of Molecular Biology and the subsequent need to desalt nucleic acids (DNA and RNA) samples, the behavior of dialysis membranes with DNA/RNA, and their high cleaness has become important.

Nucleic acids are routinely characterized by the length in base (single-stranded: ss) or pairs (double-stranded: ds), and they display far less tertiary structure than proteins. Choosing the appropriate membrane according to its retention of DNA, by length, provides a superior alternative to using the MW of the DNA fragment. The table below compares the nominal membrane MWCO to retention of various lenghts of DNA (ss and ds).

Technical Sheet

Nominal Filter rating

-

% of retention

-

-

15-mer

35-mer

117-mer

% of retention - Single stranded DNA

2.000

100 %

95 %

93 %

5.000

95 %

93 %

 90 %

10.000

50 %

82 %

82 %

15.000

50 %

82 %

82 %

25.000

39 %

74 %

68 %

% of retention - Double standed DNA

2.000

94 %

100 %

100 %

5.000

93 %

100 %

100 %

10.000

91 %

100 %

100 %

15.000

90 %

95 %

100 %

25.000

82 %

86 %

100 %

MWCO of 2.000 and 5.000 permit desalting any size fragment (ss or ds).

However, 10, 15 and 20.000 MWCO membranes provide a faster alternative when dialysing double-stranded nucleic acids.

CelluSep H1 tubings in Lenght of 50cm

MWCO

Flat width

Ø dry 

Vol/cm

Cat.# (5U)

Cat.# (10U)

1 000

18 mm

11.5 mm

1.04

H1-18-5

H1-18-10

1 000

38 mm

24.2 mm

4.59

H1-38-5

H1-38-10

1 000

45 mm

28.6 mm

6.42

H1-45-5

 H1-45-10

2 000

18 mm

11.5 mm

1.04

H2-18-5

H2-18-10

2 000

38 mm

24.2 mm

4.59

H2-38-5

H2-38-10

2 000

45 mm

28.6 mm

6.42

H2-45-5

H2-45-10

5 000

18 mm

11.5 mm

1.04

H5-18-5

H5-18-10

5 000

20 mm

12.7 mm

 1.27

H5-20-5

H5-20-10

5 000

32 mm

19.1 mm

2.86

H5-32-5

H5-32-10

5 000

40 mm

25.5 mm

5.10

H5-40-5

H5-40-10

10 000

10 mm

6.37 mm

0.32

H10-10-5

H10-10-10

10 000

25 mm

15.9 mm

1.98

H10-25-5

H10-25-10

10 000

32 mm

20.4 mm

3.27

H10-32-5

H10-32-10

10 000

40 mm

25.5 mm

5.10

H10-40-5

H10-40-10

15 000

10 mm

6.37 mm

0.32

H15-10-5

H15-10-10

15 000

25 mm

15.9 mm

1.98 

H15-25-5

H15-25-10

15 000

32 mm

20.4 mm

3.27

H15-32-5

H15-32-10

15 000

45 mm

28.6 mm

6.42

H15-45-5

H15-45-10

25 000

12 mm

7.62 mm

0.46

H25-12-5

H25-12-10

25 000

25 mm

15.9 mm

1.98

H25-25-5

H25-25-10

25 000

35 mm

22.3 mm

3.91

H25-35-5

H25-35-10

25 000

40 mm

25.5 mm 

5.10

H25-40-5

H25-40-10

CelluSep tubings H1 in Rolls

MWCO

Flat width

Ø dry 

Vol/cm

Cat.# (5m)

Cat.# (10m)

3 500

19 mm

-

1.15

-

HR03-19-10

3 500

46 mm

-

-

-

HR03-46-10

8 000

12 mm

-

0.46

HR08-12-5

HR08-12-10

8 000

24 mm

-

1.67

HR08-24-5

HR08-24-10

8 000

32 mm

-

3.27

HR08-32-5

HR08-32-10

8 000

40 mm

-

5.10

HR08-40-5

HR08-40-10

8 000

50 mm

-

7.95

HR08-50-5

HR08-50-10

10 000

12 mm

-

0.46

HR10-12-5

HR10-12-10

10 000

24 mm

-

 1.67

HR10-24-5

HR10-24-10

10 000

32 mm

-

3.27

HR10-32-5

HR10-32-10

10 000

45 mm

-

6.42

HR10-45-5

HR10-45-10

10 000

50 mm

-

7.94

HR10-50-5

HR10-50-10

15 000

12 mm

-

0.46

HR15-12-5

HR15-12-10

15 000

25 mm

-

1.98

HR15-25-5

HR15-25-10

15 000

34 mm

-

3.46

HR15-34-5

HR15-34-10

15 000

45 mm

-

6.42

HR15-45-5

HR15-45-10

 

Cellu.Sep® membrane closure clamps

Macro filler funnels

These clamps seal the tubing by a simple pressure and remain locked during dialysis until the user opens it.

They avoid the hassle of making and checking nods, reduce the risk of loosing sample.

The clamps are made of resistant and inert polyamide 6.6 (NYLON), and do not float, for an optimal dialysis.

Cut tubular membrane to length and clamp at one end, slip the open end onto the polypropylene funnel and secure with the silicone loop fastener.

Open snap cap, fill with sample, and hang in container. Funnels are supported by a three-notched universal hanger that fits over the edge of common vessels.  

Description - max membrane widht

Lenght

Cat. #

Qty

Locking clamp - 46 mm

65mm

CB-1050

10U

Locking clamp - 65 mm

90mm

CB-1070

10U

Locking clamp - 105 mm

130mm

CB-10100

10U

Description

Cat. #

Qty

Macro filler funnel

P58480

5U

For 23 mm, 25 mm, 32 mm, 40 mm and 45 mm flat widths.

Package of five funnels and two silicone loops.

Mini filler funnel

P58490

5U

For 10 mm and 19 mm flat widths.

Package of five funnels and two silicone loops

Silicone loops

P58500

10U

 

BACK

 

211 Bis Avenue Kennedy - BP 1140 - 03103  Montluçon cedex - FRANCE

Tel (33) (0) 4 70 03 88 55 -  Fax (33) (0) 4 70 03 82 60 - uptima@interchim.com  www.interchim.com