Membranes CelluSep

Desalting

DIALYSIS / DESALTING

Dialysis with CelluSep Membranes

Introduction to CELLUSEP Dialysis Products

Cellu•Sep® Dialysis tubings are the best choice for dialysis, notably in Biology applications, for proteins and nucleic acids. They are regenerated cellulose membranes manufactured with high quality process for optimal and reproducible results

T1, T2, T3 and T4 Cellu•Sep® Dialysis Membranes

suit classical dialysis applications, especially when attention is paid to very low absorption of proteins, low impurity levels , good compatibilities, and economic costs

H1 Cellu•Sep® Dialysis Membranes

suit most exigent applications (minimal contaminants content, ready-to-use individual packages); suit notably DNA desalting

Cellu.Sep membrane closure clamps

Macro Filler funnel - Mini Filler funnel - Silicone loops

Ask for any questions :

Sterilisation of membranes
Cleaning of heavy metals and sulfides
Concentrating sample directly in your dialysis bag!

Cellu•Sep® membranes Performances
Protein absorption
(bovine serum albumin)	<=1mg/cm²
Retention
(T=10 hours, with T2 Membranes)
Cytochrome C (12,3 KDa)	97%
Vitamin B-12 (1355 Da)	59%

Cellu• Sep® T1, T2, T3 and T4 tubular dialysis membranes

Cellu•Sep® T1, T2, T3 et T4 membranes suit most dialysis applications

Very low absorption of proteins
Pure regenerated cellulose for low impurity levels
Nominal Filter ratings : 3500, 6000 and 12000 Daltons
Economical

Each package includes :

A detailled instruction manual
Two clamps

Cellu•Sep® membranes technical data
Cadmium	< 0.1 ppm
Chromium	< 0.1 ppm
Coper	< 0.1 ppm
Iron	13.6 ppm
Nickel	< 0.1 ppm
Zinc	6.24 - 12.9 ppm
Lead	0.75 - 0.8 ppm
Cellu•Sep® T1, T2, T3 et T4 membranes contain glycerol to prevent brittleness, and are packaged with a dessicant to control humidity.

Technical Sheet

Cellu•Sep® T1 Membranes Nominal Filter rating : 3500 Da (4-6000 MWCO)
Flat width	Ø dry	Vol/cm	Cat. #	Lenght
19 mm	12.1 mm	1.15	T1-19-15	15 m
46 mm	29.3 mm	6.74	T1-46-15	15 m

Cellu•Sep® T3 Membranes Nominal Filter rating : 12000 Da (12-14000 MWCO)
Flat width	Ø dry	Vol/cm	Cat. #	Lenght
10 mm	6.37 mm	0.32	T3-10-15	15 m
25 mm	15.9 mm	1.98	T3-25-15	15 m
33 mm	21.0 mm	3.46	T3-33-15	15 m
45 mm	28.6 mm	6.42	T3-45-15	15 m
76 mm	48.4 mm	18.4	T3-76-5	5 m

Cellu•Sep® T2 Membranes Nominal Filter rating : 6000 Da (6-8000 MWCO) ----------
Flat width	Ø dry	Vol/cm	Cat. #	Lenght
10 mm	6.37 mm	0.32	T2-10-15	15 m
23 mm	14.6 mm	1.67	T2-23-15	15 m
25 mm	15.9 mm	1.98	T2-25-15	15 m
32 mm	20.4 mm	3.27	T2-32-15	15 m
40 mm	25.5 mm	5.10	T2-40-15	15 m
50 mm	31.8 mm	7.94	T2-50-15	15 m

Cellu•Sep® T4 Membranes Nominal Filter rating : 12000 Da (12000 MWCO) Standard grade (with accessories without technical sheet)
Flat width	Ø dry	Vol/cm	Cat. #	Lenght
10 mm	6.37 mm	0.32	T4-10-30	30 m
25 mm	15.9 mm	1.98	T4-25-30	30 m
33 mm	21.0 mm	3.46	T4-33-30	30 m
45 mm	28.6 mm	6.42	T4-45-30	30 m

Cellu•Sep® H1 Membranes

High Grade

suit protein applications, and especially DNA/RNA applications

Pre-treated for minimal contaminants content
Ready-to-use individual-use packaged
MWCO from 1 000 to 25 000 Daltons

Cellu•Sep® H1 are ideal for sensitive applications where glycerol, sulfur compounds, or small amounts of heavy metals will interfere with subsequent steps. Cellu•Sep® H1 membranes are EDTA pretreated and packaged wet in a preservative solution. Individual-use 50cm packages avoid waste, contamination and restorage problems.

Cellu•Sep® H1 provide a efficient tool for purifying DNA and RNA, allowing both dialysis and concentration (ask Uptima).

For proteins, notably globular ones, Molecular Weight Cut-Off (MWCO) has become a convenient guide for determining wich membrane to use. Ultimately, however the size and the three-dimensionnal shape of a molecule determine wether it pass or not through a membrane.
With the important expansion of Molecular Biology and the subsequent need to desalt nucleic acids (DNA and RNA) samples, the behavior of dialysis membranes with DNA/RNA, and their high cleaness has become important.

Nucleic acids are routinely characterized by the length in base (single-stranded: ss) or pairs (double-stranded: ds), and they display far less tertiary structure than proteins. Choosing the appropriate membrane according to its retention of DNA, by length, provides a superior alternative to using the MW of the DNA fragment. The table below compares the nominal membrane MWCO to retention of various lenghts of DNA (ss and ds).

Technical Sheet

Nominal Filter rating	-	% of retention	-
-	15-mer	35-mer	117-mer
% of retention - Single stranded DNA
2.000	100 %	95 %	93 %
5.000	95 %	93 %	90 %
10.000	50 %	82 %	82 %
15.000	50 %	82 %	82 %
25.000	39 %	74 %	68 %
% of retention - Double standed DNA
2.000	94 %	100 %	100 %
5.000	93 %	100 %	100 %
10.000	91 %	100 %	100 %
15.000	90 %	95 %	100 %
25.000	82 %	86 %	100 %

MWCO of 2.000 and 5.000 permit desalting any size fragment (ss or ds).

However, 10, 15 and 20.000 MWCO membranes provide a faster alternative when dialysing double-stranded nucleic acids.

CelluSep H1 tubings in Lenght of 50cm
MWCO	Flat width	Ø dry	Vol/cm	Cat.# (5U)	Cat.# (10U)
1 000	18 mm	11.5 mm	1.04	H1-18-5	H1-18-10
1 000	38 mm	24.2 mm	4.59	H1-38-5	H1-38-10
1 000	45 mm	28.6 mm	6.42	H1-45-5	H1-45-10
2 000	18 mm	11.5 mm	1.04	H2-18-5	H2-18-10
2 000	38 mm	24.2 mm	4.59	H2-38-5	H2-38-10
2 000	45 mm	28.6 mm	6.42	H2-45-5	H2-45-10
5 000	18 mm	11.5 mm	1.04	H5-18-5	H5-18-10
5 000	20 mm	12.7 mm	1.27	H5-20-5	H5-20-10
5 000	32 mm	19.1 mm	2.86	H5-32-5	H5-32-10
5 000	40 mm	25.5 mm	5.10	H5-40-5	H5-40-10
10 000	10 mm	6.37 mm	0.32	H10-10-5	H10-10-10
10 000	25 mm	15.9 mm	1.98	H10-25-5	H10-25-10
10 000	32 mm	20.4 mm	3.27	H10-32-5	H10-32-10
10 000	40 mm	25.5 mm	5.10	H10-40-5	H10-40-10
15 000	10 mm	6.37 mm	0.32	H15-10-5	H15-10-10
15 000	25 mm	15.9 mm	1.98	H15-25-5	H15-25-10
15 000	32 mm	20.4 mm	3.27	H15-32-5	H15-32-10
15 000	45 mm	28.6 mm	6.42	H15-45-5	H15-45-10
25 000	12 mm	7.62 mm	0.46	H25-12-5	H25-12-10
25 000	25 mm	15.9 mm	1.98	H25-25-5	H25-25-10
25 000	35 mm	22.3 mm	3.91	H25-35-5	H25-35-10
25 000	40 mm	25.5 mm	5.10	H25-40-5	H25-40-10
CelluSep tubings H1 in Rolls
MWCO	Flat width	Ø dry	Vol/cm	Cat.# (5m)	Cat.# (10m)
3 500	19 mm	-	1.15	-	HR03-19-10
3 500	46 mm	-	-	-	HR03-46-10
8 000	12 mm	-	0.46	HR08-12-5	HR08-12-10
8 000	24 mm	-	1.67	HR08-24-5	HR08-24-10
8 000	32 mm	-	3.27	HR08-32-5	HR08-32-10
8 000	40 mm	-	5.10	HR08-40-5	HR08-40-10
8 000	50 mm	-	7.95	HR08-50-5	HR08-50-10
10 000	12 mm	-	0.46	HR10-12-5	HR10-12-10
10 000	24 mm	-	1.67	HR10-24-5	HR10-24-10
10 000	32 mm	-	3.27	HR10-32-5	HR10-32-10
10 000	45 mm	-	6.42	HR10-45-5	HR10-45-10
10 000	50 mm	-	7.94	HR10-50-5	HR10-50-10
15 000	12 mm	-	0.46	HR15-12-5	HR15-12-10
15 000	25 mm	-	1.98	HR15-25-5	HR15-25-10
15 000	34 mm	-	3.46	HR15-34-5	HR15-34-10
15 000	45 mm	-	6.42	HR15-45-5	HR15-45-10

Cellu.Sep® membrane closure clamps

Macro filler funnels

These clamps seal the tubing by a simple pressure and remain locked during dialysis until the user opens it.

They avoid the hassle of making and checking nods, reduce the risk of loosing sample.

The clamps are made of resistant and inert polyamide 6.6 (NYLON), and do not float, for an optimal dialysis.

Cut tubular membrane to length and clamp at one end, slip the open end onto the polypropylene funnel and secure with the silicone loop fastener.

Open snap cap, fill with sample, and hang in container. Funnels are supported by a three-notched universal hanger that fits over the edge of common vessels.

Description - max membrane widht	Lenght	Cat. #	Qty
Locking clamp - 46 mm	65mm	CB-1050	10U
Locking clamp - 65 mm	90mm	CB-1070	10U
Locking clamp - 105 mm	130mm	CB-10100	10U

Description	Cat. #	Qty
Macro filler funnel	P58480	5U
For 23 mm, 25 mm, 32 mm, 40 mm and 45 mm flat widths. Package of five funnels and two silicone loops.
Mini filler funnel	P58490	5U
For 10 mm and 19 mm flat widths. Package of five funnels and two silicone loops
Silicone loops	P58500	10U

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Tel (33) (0) 4 70 03 88 55 - Fax (33) (0) 4 70 03 82 60 - uptima@interchim.com www.interchim.com