nucleic acid purification
UptiDNApure PCR and others reactions Clean-up Kit
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Molecular Biology |
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nucleic acid purification UptiDNApure PCR and others reactions Clean-up Kit |
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After biological reactions, such as PCR, fluorescent labeling, cloning and sequencing, etc, the unused materials, such as primers, enzymes, mononucleotides, florescenceins and radioisotope-labeled mononucleotides and salts should be removed. PCR Clean-up Kit is designed for rapid purification of DNA fragments from PCR samples or other reactions. |
Agarose gel (1.6%) analysis of PCR products before (lane 1, 3, 5, 7) and after (lane 2, 4,6 ,8) purification with the UptiDNApure PCR Clean-up |
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Principle and Procedure |
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Buffer PCR-A contains high concentration of chaotropic salt, which allows DNA to selectively bind to silica membrane. After addition of Buffer PCR-A to PCR samples or other enzymetic reactions, the binding mixture is loaded to DNA-prep tube. After centrifugation or vacuum drawing, more than 100 bp DNA fragments bind to the membrane and impurities are removed by ashing with Buffer W1 and Buffer W2. The purified DNA fragments are then eluted in small volume of water or Tris buffer. |
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Applications |
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PCR Clean-up Kit is suitable for purification of DNA fragments used for sequencing, ligation, micro-injection, DNA microarry-based analysis. |
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UptiDNApure PCR and others reactions Clean-up Kit |
50 tests |
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250 tests |
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211 Bis Avenue Kennedy - BP 1140 - 03103 Montluçon cedex - FRANCE Tel (33) (0) 4 70 03 88 55 - Hot Line (33) (0) 4 70 03 73 06 - Fax (33) (0) 4 70 03 82 60 |
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