X-GAL

UP40534M

1g

5x1g

Substrate for reporter gene b-Galactosidase detection

5-Bromo-6-Chloro-3-Indolyl-b-D-Galactopyranoside

C₁₄H₁₅BrClNO₆

CAS [7240-90-67]

MW 408.6

DNAse, Rnase free

Bluo-Gal is used to detect the b-galactosidase enzyme. It produces a blue insoluble precipitate. It is used notably in histochemistry detections and blotting. It is also used with IPTG (UP84853) to distinguish recombinant plasmids of detect fusion proteins that are tagged with the coding lac z gene coding for the b-galactosidase enzyme.

Applications

Rapid detection of coliform bacteria in water or food with a minimal false positives and false negatives (6.7)

Distinction between bacterriophages and parental vectors (2.3.5)

Detection of lac z gene and identification of colonies (2.3.5)

Localisation of b-galactosidase enzymatic activity in tissues sections

Detection of clonage inserts in histochemistry (2.3.5).

1. Malfoy 1990

2. Miller 1992

3. Sambrook 1989

4. Horowitz 1964

5. Brand 1993

6. Ley 1993

7. Feldsine 1993

ONPG

UP556683

5g

25g

Substrate for the b-Galactosidase

o-NitroPhenyl-b-D-Galactopyranoside

C₁₂H₁₅NO₈

MW 301.25

Produces a yellow soluble compound Substrate for the b-Galacto-sidase

UP775600

5g

UP775601

25g

Substrate for the b-Galactosidase

p-NitroPhenyl-b-D-Galactopyranoside

C₁₂H₁₅NO₈

MW 301.25

Bluo-GAL

UP775580

1g

Biotechnology grade

Substrate for reporter gene b-Galactosidase detection

5-Bromo--3-Indolyl-b-D-Galactopyranoside

C₁₄H₁₆BrNO₆

CAS [97753-82-7]

MW 374.2

Bluo-Gal is used to detect the b-galactosidase enzyme, used notably to tag fusion protein expressing cells, through the coding lac z gene.

IPTG

UP84853C

1g

Molecular Biology grade

UP84853D

10x1g

Lac+ inducer for the criblage of E.coli

• Purity >99%

• Dioxane free

• RNase, DNAse, Proteases free

Isopropyl-b-D-thioGalactoPyranoside

C₉H₁₈O₅S

CAS [367-93-1]

MW  238.3

IPTG induces the expression of lac+ lactose operon, as it’s analog galactose, but is not hydrolysed by b-galactosidase. Used with X-GAL(UP40534), IPTG alow the detection of recombinant bacteria that form blue colonies on gelose media.

X-GLUCcha

UP194285

100mg

Biotechnology grade

Substrate of b-Glucuronidase

5-Bromo-6-Chloro-3-Indolyl-b-D-Glucuronic acid, cyclohexylamonium salt

C₂₀H₂₆BrClN₂O₇

CAS [1114162-64-0]

MW 521.8

• Gain of time compared with other detection methods on E.coli (24hours, direct method in plate) (7.9.10)

• Less than 1% false positive, less than 5% of false negative (7.8.9)

• Overcome limitations encountered with chemiluminescent methods (11)

X-GLU detect the b-glucuronidase enzyme activity. After reduction, X-GLU produces a colored precipitate easily detectable. It is used notably for the detection of GUS gene in most of cell types.

Technical sheet

Applications

E.coli testing in water (7.8.9.11)

Detection of GUS gene expression in transcription, Traduction,and protein transfert studies (2.3)

Urinary infectious determination (6)

Identification of E.coli contaminated food (4.5)

1. Ellis 1993

2. Bommineni 1993

3. Martin 1990

4. Restaino 1990

5. Watkins 1988

6. Delisle 1989

7. Gaudet 1996

8. Ceibin 1995

9. Sartory 1992

10. Frampton 1988

11. Van Pouckld 1997

X-GLUC, Na

UPM16321

100mg

Substrate of b-Galactosidase

5-Bromo-4-Chloro-3-Indolyl-b-D-Glucuronic acid, sodium salt

C₁₄H₁₂BrClNO₇Na

CAS [129541-41-9]

MW 444.6

Technical sheet

UP248742

1g

Substrate of b-Galactosidase

4-Methylumbelliferyl-b-D-Glucuronic Acid

C₁₆H₁₆O₉

CAS [6160-80-1]

MW 352.3

Criblage of E.coli

UP193325

100mg

Substrate of b-Galactosidase

5-Bromo-4-Chloro-3-Indolyl-b-D-Glucopyranoside

C₁₄H₁₅BrClNO₆

CAS [15548-60-4]

MW 408.6