CROSS-LINKERS

See also: Protein modifyers - Buffers - Labeling reagents (biotin)

1

2

3

4

5

6

7

8

9

10

11

12

13

Photoreactive cross-linkers have the particularity that chemical reactivity is induced by illumination. They are original tools useful when cross-linking should occur in a defined site (organ, cell, compartment) or in a defined in a defined time, or when classic reactivities are not working. The reaction is effectively not specific, working with almost all molecules containing Hydrogens :

• physiological studies

• conjugates of difficult molecules (steroids...)

Photo-reactive cross-linkers / homobifunctional (page10...page13)

DADPEthane

BASED

DADPEthane

UPG6673A

On Inquire

DADPEther

UPG6674A

On Inquire

Non specific conjugation

• Phenyl azides react non-specifically with 2 biomolecules

Non specific conjugation

• Phenyl azides react non-specifically with 2 biomolecules

4,4'-DiAzidoDiPhenyl-Ethane

MW 252.2

4,4'-DiAzidoDiPhenyl-Ether

MW 264.3

Applications 

Structural studies

Applications / Literature

The original O-spacer may be useful for the study of sterically restricted sites

BASED

UP67018A

100mg

DTPA

UP63972A

100mg

UP67018B

Non specific photoreactive and cleavable cross-linker

• Reacts non-specifically with 2 biomolecules

• Extended 34.7 A spacer

• Cleavable by thiol reducing agents

• Features of the BASED

• Short spacer

Bis(b-(4-AzidoSalicylaminoEthyl)Disulfide

MW 474.5

Dithio bis Phenyl Azide

MW 300.4

CAS [37434-06-3]

Applications / Literature

Non specific conjugation for biomolecules difficult to handle

Hermanson (1996)

Applications / Literature

Mikkelsen (1976), Sakai (1989)

1

2

3

4

5

6

7

8

9

10

11

12

13

BACK

211 Bis Avenue Kennedy - BP 1140 - 03103  Montluçon cedex - FRANCE

Tel (33) (0) 4 70 03 88 55 -  Fax (33) (0) 4 70 03 82 60 - uptima@interchim.com  www.interchim.com