Biochemistry of Proteins

CROSS-LINKERS

See also: Protein modifyers - Buffers - Labeling reagents (biotin)

 

 

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Photo-reactive cross-linkers with amine reactivity (page10...page13)

Sulfo-SANPAH

Sulfo-SAPB

Sulfo-SAH

HSAB
Sulfo-HSAB

SASD

APG

NHS-ASA

 

Sulfo-SANPAH

UP09649A

100mg

Sulfo-SAPB

UP34514A

100mg

UP09649B

50mg

Extended and flexible spacer

  • SulfoNHS reacts with amines at pH6.5-9.5

  • Nitrophenyl azide reacts with amines upon photolysis at 320nm-350nm

  • Low alteration of biomolecules

  • 18.2nm linear chain spacer

Shorter spacer than Sulfo-SANPAH

  • SulfoNHS reacts with amines at pH6.5-9.5

  • Nitrophenyl azide reacts with amines upon photolysis

  • 4 C-long linear chain spacer

SulfoSuccinimidyl-6(4'-azido-2'-nitrophenylamino)hexanoate

MW 492.4

SulfoSuccinimidyl-4(p-azidophenyl)butyrate

MW 403.2

Applications / Literature

In situ membrane receptor/ligand studies

Wood (1985), Ballmer-Hofer (1981), Schmitt (1983)

Applications / Literature

In situ membrane receptor/ligand studies

Sulfo-SAH

UPG9975A

100mg

-

Analog to Sulfo-SANPAH

  • SulfoNHS reacts with amines at pH6.5-9.5

  • Iodinatable

  • Hydroxyphenyl azide reacts with amines upon photolysis

  • 8 C-long linear chain spacer

SulfoSuccinimidyl-(4-azidoSalicylamido)hexanoate

MW 491.4

Applications / Literature

Ji (1982)

HSAB

UPJ9107A

 On inquire

Sulfo-HSAB

UP05006A

100mg

UP05006B

50mg
Photoreactive and amine reactive cross-linker

  • Succinmidyl group reacts specifically with amines at pH6.5-9.5

  • Phenyl azide reacts non specifically upon photolysis at 250 nm

  • 9.0 Å spacer

The water soluble analog of HSAB

  • Feature of the HSAB

N-HydroxySuccinimidyl-4-azido benzoate

MW 260.2

CAS [53053-08-0]

N-HydroxySulfo Succinimidyl-4-azido benzoate

MW 362.2

Applications / Literature

Photo affinity labeling of peptide - hormone binding site Galardy (1974)

Vandlen (1985)

Applications / Literature

Membrane receptor studies

Galardy (1974), Ballmer-Hofer (1982), Wood (1985)

SASD

UP40901B

 100mg

APG

UP28071A

100mg

UP40901A

50mg

UP28071B

50mg
Photoreactive, iodinatable and cleavable !

  • SulfoNHS ester reacts specifically with amines at pH7-10

  • Hydroxyphenyl azide reacts with amines upon UV 265-275nm photolysis

  • 13.9 Å spacer, cleavable by thiols reducing agents

  • Soluble directly in aqueous buffer (no need DMSO)

  • Do not cross biological membranes

Photoreactive and arginine selective

  • Phenylglyoxal reacts with arginine residues at pH7-8

  • Phenyl azide reacts amines upon light photolysis

  • 9.3 Å rigid spacer

Sulfosuccinimidyl-2-(p-AzidoSalicylamido)-ethyl-1,3-dithiopropionate

MW 541.5

p-AzidoPhenyl Glyoxal, monohydrate

MW 193.2

CAS [96602-46-9]

Applications / Literature

Ligand interactions studies, especially with radiolabeling techniques

Ji (1982), Sorenson (1986), Chattopadhyay (1992), Lala (1996)

Applications / Literature

Ngo (1981), Sgro (1986)

Technical Sheet

NHS-ASA

UP42252B

 100mg

-

UP42252A

50mg

SulfoNHS ester reacts specifically with amines at pH7-10

  • NHS reacts with amines at pH7-9

  • Hydroxyphenyl Azide reacts with amines upon long wavelenght photolysis

  • 8.0 Å rigid spacer

N-HydroxySuccinimidyl-4-azidoSalicylic acid

MW 276.2

Applications / Literature

Ligand interactions studies, especially with radiolabeling techniques

Shanadan (1985), Ji (1985), Van der Horst (1990)

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