CROSS-LINKERS

See also: Protein modifyers - Buffers - Labeling reagents (biotin)

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Heterobifunctional cross-linkers SH / Halogens -- NHS / NH2 (page 2...page6)

SIAB

Sulfo-SIAB

NHS-BA (Bromoacetate)

NHS-IA (Iodoacetate)

SIAB

UPG9906A

100mg

Sulfo-SIAB

UP75036A

100mg

UPG9906B

50mg

UP75036B

50mg

Alternative to SMCC

• Reacts with sulfhydryls (through iodoacetate) and amines (through NHS)

• Maleimide stabilized by the cyclohexane ring

• Iodinatable

• 10.6 Å spacer

Water soluble analog of SIAB

• Features of SIAB

• Soluble directly in aqueous buffer (no need DMSO)

• Do not cross biological membranes

Succinimidyl-4-iodoacetyl-AminoBenzoate

MW 402.2

SulfoSuccinimidyl-4-iodoacetyl-AminoBenzoate

MW 504.2

Applications / Literature

Immuno-conjugates: toxin-antibodies, Cumber (1985)

To Hermanson (1996), Weltman (1983)

Applications / Literature

Weltman (1983), Hermansson (1996)

NHS-BA

UPG9908A

1g

NHS-IA

UPG9907B

1x1g

(Bromoacetate)

(Iodoacetate)

UPG9907A

1x500mg

Alternative to SMCC

• Reacts with sulfhydryls (with bromoacetate) and amines (with succinimidyl)

Reacts with sulfhydryls (with bromoacetate) and amines (with NHS)

N-hydroxySuccinimidyl—bromoacetate

MW 236.0

CAS [42014-51-7]

N-hydroxySuccinimidyl—iodoacetate

MW 283

CAS [39028-27-8]

Applications / Literature

Immuno-conjugates: Bernatowicz (1986), toxin-antibodies, Cumber (1985)

Cuatrecasas (1969)

Applications / Literature

Rector (1978)

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