CROSS-LINKERS

See also: Protein modifyers - Buffers - Labeling reagents (biotin)

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Heterobifunctional cross-linkers NH2 / carbodiimide / HOOC (page 2...page6)

EDAC

NHS

Sulfo-NHS

EDAC

UP520059

100mg

Complementary Products

UP52005A

50mg

NHS

UP04594A

500mg

50mg

-

UP04594B

5g

50mg

Sulfo-NHS

UP54422A

500mg

A classic crosslinker for peptidic crosslinking

• Reacts with amines and carbonyls after the formation of an intermediate complex

• Formation of a stable peptidic bond

• Enhance reaction with NHS (UP04594A )

• Widely used in biosynthesis, peptide conjugation to carriers

Increases protein/peptide conjugation by EDC

• Activates -COOH to form an amide bond

• Sulfo-NHS is water soluble

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1-Ethyl-3-(3DimethylAminopropyl)Carbodiimide

MW 191.7

Applications / Literature

Taniuchi (1986), Greenfield (1990)

Sulfo-NHS

N-HydroxySulfoSuccinimide

MW 217.1

N-HydroxySuccinimide

MW 115.1

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