Biochemistry of Proteins

CROSS-LINKERS

See also: Protein modifyers - Buffers - Labeling reagents (biotin)

 

 

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Heterobifunctional cross-linkers CHO / hydrazide -- maleimide (page 2...page6)

MPH

MCH (EMCH)

PDPH

KMUH

MPITC (PMPI)

MPBH

 

MPH

UPG9909A

50mg

MCH(EMCH)

UPG9910A

50mg

SH and carbohydrate reactive crosslinker

  • Reacts with sulfhydryls (through maleimide) and carbohydrates (through hydrazide)

  • Linear 4C spacer

Extended chain version of MPH

  • Reacts with sulfhydryls (through maleimide) and carbohydrates (through hydrazide)

  • Linear 6C spacer

MaleimidoPropionic acid Hydrazide.HCl

MW 233.65

e-MaleimidoCaproic acid Hydrazide.HCl ester

MW 261.8

 

Technical Sheet

Applications / Literature

Conjugates antibodies through Fc carbohydrate to Cys-containing peptides

Technical Sheet

Applications / Literature

Trail ( 1993)

PDPH

UP99648A

50mg

KMUH

UPL7722A

100mg
UPL7722B 50mg

Alternative to MPH, and cleavable

  • Reacts with sulfhydryls (through pyridylthiol) and carbohydrates (through hydrazide)

  • Linear 7-atoms spacer

  • Easily cleavable by reducing agents

Extended chain version of MPH

  • Reacts with sulfhydryls (through maleimide) and carbohydrates (through hydrazide)

  • 19.0 Å linear and flexible spacer

3-(2-pyridyldithio)-propionic acid Hydrazide.HCl

MW 279.81

 

 

N-(k-Maleimidoundecanoic acid)hydrazide

 

MW 295.4

 

Technical Sheet

Applications / Literature

Trail ( 1993)

MPITC(PMPI)

UP88307A

50mg

MPBH

UP09835A

100mg
UP09835B 50mg

SH and OH reactive crosslinker: useful for protein to steroid or carbohydrate conjugation

  • Maleimide reacts with SH at pH6.5-7.5 forming a thioether bond

  • Isothiocyanate reacts with nucleophil (a confirmer TD) OH, particularly hydroxyls at pH8.5

  • 8.7 Å rigid spacer

A unique SH and CHO reactive crosslinker

  • Maleimide reacts with SH at pH6.5-7.5 forming a thioether bond

  • Hydrazide reacts with Carbohydrates

  • 17.9 Å spacer

N-(p-MaleimidoPhenyl)IsoThioCyanate

MW 214.1

4-(4-N-MaleimidoPhenyl)butyric acid Hydrazide.HCI

MW 309.8

Applications / Literature

Conjugates between proteins and steroids, toxins, or glycosylated lipids

Annunziato (1993), Palumbo (1993)

 

Technical Sheet

Applications / Literature

Oriented conjugates between proteins and saccharides, or glycosylated lipids

Chamow (1992)

 

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