Homobifunctional Cross-linkers

Biochemistry of Proteins

CROSS-LINKERS

Homobifunctional cross-linkers contain 2 identic chemical reactif groups. Dimeres (but also some tri- and oligomeres) are generally formed besides conjugates of same molecules, or different molecules. Homobifunctional cross-linkers are dedicated mostly for coupling same molecules together or to immobilization, for production and special studies :

immobilization of ligands to supports (plates, gels, beads)
production of polymeres at big scale (antigens...)
reticulation of big molecules / complexes, and membranes

design of multimeres for the study of natural polymeres...

Homobifunctional cross-linkers NH2/HNS -- NHS / NH2 (page7...page9)

DSS

Sulfo-DSS(BS3)

100mg

5x1g

50mg

A classical homobifunctional amine reactive crosslinker

Reacts with NH2 via NHS at pH7-9 forming a stable amide bond
11.4Å linear spacer, very flexible, non cleavable

Water soluble analog of DSS

Features of the DSS
Soluble directly in aqueous buffer (no need DMSO)
Do not cross biological membranes

Dissucinimidyl Suberate

MW 368.4

CAS [68528-80-3]

Bis(Sulfosuccinimidyl) Suberate ester

MW 572.4

CAS [82436-77-9]

Technical Sheet

Applications / Literature

Receptor-protein studies

Immobilisation of IgG on protein A

Cox (1990), Petruzelli (1985), Rashidbaigi (1986), Sawyer (1987)

Technical Sheet

Applications / Literature

Cell Crosslinking biomolecules on cells (Jordan 1996)

In Out-side membrane receptor-protein studies

Bifunctional bioconjugates

Wolf (1994), Locascio-Brown (1990), Terlecky (1996),

Jordan(1996), Sargiacome (1995), Knoller (1991),

Staros (1982), Ward (1985), Souza (1988)

Arylated spacer should be useful when hydrophobic patterns, or when other spacer are though to be short for the desired crosslinking.

DMAI(DMA)	UP09962A	1g	DMPI(DMP)	UP362009	100mg
	UP09962B	10g		UP36200A	1g
				UP36200B	10g
An original alternative to DSS and DSG Reacts with NH2 via imidoesters at pH8-10 forming a charged amide bond Short 8.6Å spacer (6 atoms) Cleavable at pH>10			Slightly longer spacer than DMAI Features of the DMAI Short 9.2Å spacer (7 atoms)
Dimethyl Adipimidate.2HCl MW 245.1 CAS [14620-72-5]			Dimethyl Pimelimidate.2HCl MW 259.2 CAS [58537-94-3] Protein-protein interaction studies
Technical Sheet Applications / Literature Protein-protein interaction studies Hartman (1967)			Technical Sheet Applications / Literature Protein confirmation studies Brew (1975) Schneider (1982)

DMSI(DMS)	UP06633A	1g	DTBP	UP997960	On inquire
	UP06633B	10g
Slighly longer spacer than DMPI Features of the DMAI Short 11.0Å spacer (8 atoms)			The cleavable analog of DMSI Features of the DMAI Thiol-cleavable 11.9Å spacer (8 atoms)
Dimethyl Suberimidate.2HCl MW 259.2 CAS [34490-86-3]			Dimethyl-3,3'-DithioBisPropionamidate.2HCl MW 309.3 CAS [38285-78-8]
Technical Sheet Applications / Literature Protein-protein interaction studies, Darrest (1970) Wang (1977)			Applications / Literature Protein-protein interaction studies Shiudasani (1988), Wang (1974), Labbe (1982), Ruotto (1975)