Biochemistry of Proteins

CROSS-LINKERS

See also: Protein modifyers - Buffers - Labeling reagents (biotin)

 

 

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Homobifunctional cross-linkers NH2/HNS -- NHS / NH2 (cleavable) (page7...page9)

DSP(DTSP)

Sulfo-DSP(DTSSP)

BSOCOES

DST

Sulfo-DST

EGS

Sulfo-EGS

 

DSP

UP18971A

1g

Sulfo-DSP(DTSSP)

UP434320

100mg

Lomant's reagent

UP43432B

50mg

Thiol-cleavable analog of DSS

  • Reacts with NH2 via NHS at pH7-9 forming a stable amide bond

  • 12.0Å linear spacer

  • Easily cleavable by reducing agents

  • Can be used as a thiolation agent

Water soluble analog of DSP

  • Features of the DSP

  • Soluble directly in aqueous buffer (no need DMSO)

  • Do not cross biological membranes

Dithio-bis(succinimidyl Propionate

MW 404.4

CAS [57757-57-0]

3,3'-Dithiobis(sulfosuccinimidyl Propionate)

MW 608.5

Technical Sheet

Applications / Literature

Enzymatic complex studies, Joshi (1990)

Biosensor, Darder (1999)

Joshi (1990), Lomant (1976), Travers (1982)

Technical Sheet

Applications / Literature

Membran receptor complex studies, Jung (1983)

Staros (1982), Lee (1985), Ward (1985)

BSOCOES

UP28069A

100mg

Lomant's reagent

UP28069B

50mg

Base-cleavable analog of DSS

  • Suits when oxidizing conditions must be avoided (for example with metal chelates, hemes…)

  • Reacts with NH2 via NHS at pH7-9 forming a stable amide bond

  • 13.0Å spacer

  • Cleavable by alkaline conditions pH11.6

Bis(2-(SuccinimidylOxyCarbonylOxy)-Ethyl)Sulfone

MW 436.4

CAS [57683-72-4]

Technical Sheet

Applications / Literature

Enzymatic complex studies, Joshi (1990)

Biosensor, Darder (1999)

Joshi (1990), Lomant (1976), Travers (1982)

DST

UP280681

1g

Sulfo-DST

UP24864A

100mg

UP24864B

50mg

Oxidizer-cleavable homobifonctional crosslinker

  • Suits when reducing conditions are to be avoided (for example with -S-S- dependant folding)

  • Reacts with NH2 via NHS at pH7-9 forming a stable amide bond

  • 6.4Å linear spacer, shorter than DSS

  • Cleavable by alkaline conditions pH11.6 (ex: with periodate)

Water soluble analog of DST

  • Features of the DST

  • Soluble directly in aqueous buffer (no need DMSO)

  • Do not cross biological membranes

DiSuccinimidyl Tartarate

MW 344.2

DiSulfoSuccinimidyl Tartarate

MW 584.3

Technical Sheet

Applications / Literature

Farries (1989), Park (1986), Smith (1978)

Technical Sheet

Applications / Literature

Membrane receptor studies

Park (1986), Smith (1978), Bragg (1980)

EGS

UP28067A

1g

Sulfo-EGS

UP24455A

100mg

UP24455B

50mg

Extended and cleavable spacer analog of DSS

  • Reacts with NH2 via NHS at pH7-9 forming a stable amide bond

  • 16.1 linear spacer, longer than DSS

  • Cleavable by mild alkaline conditions (ex: pH8.5 with hydroxylamine)

Water soluble analog of EGS

  • Features of the DST

  • Soluble directly in aqueous buffer (no need DMSO)

  • Do not cross biological membranes

EthylGlycol bis(SuccinimidylSuccinate)

MW 456.4

CAS [70539-42-3]

EthylGlycol bis(SulfoSuccinimidylSuccinate)

MW 660.5

Technical Sheet

Applications / Literature

Related products. hydroxylamine

Receptor-ligand complexes studies

Millar (1990), Browning (1989), Abdella (1979), Conn (1982)

Technical Sheet

Applications / Literature

Membrane receptor studies

Abdella (1979), Browning (1989)

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