Molecular Biology

sequencing


 

 

Acrylamides solution

Formamide, deionized

UP070990

100ml

Ultra Pure Grade

Purity >99.5%

Conductivity <100 µmhos

RNase,DNase activity : none detected

Formamide is widely used for hybridizing and denaturing nucleic acids(1). To be suitable for such applications, formamide must be highly deionized and free of contaminants. Ultra Pure Formamide possesses a high degree of purity and is free of contaminating RNase and DNase making it the ideal choice for all molecular biology applications. It is suitable for the preparation of hybridization cocktails and various denaturing solutions used with nucleic acids. It will not cause degradation of expensive or hard to isolate RNA and DNA samples. Each lot of material is pre-filtered through a mixed-ion exchange resin to eliminate products of decomposition and residual metal cations. This filtering process guarantees that each lot has a final purity of greater than 99.5% and an absorbance value of less than 0.05 absorbance units at 280nm. Each lot then undergoes rigid quality control testing to insure that assay specifications are met prior to release.

Literature:

1. Ausubel, F.M., Brent, R., Kingston, R.E., Moore, D.D., Seidman, J.G., Smith, J.A., Stroll, K. 1992. Current Protocols In Molecular Biology. (1)6.3.1

Ammonium Persulfate

UptiTherm EC DNA Polymerase

dNTP

TBE

SDS

Urea

 

 

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