Biochemistry of Proteins

IMMUNIZATION

TiterMax™ Immunization adjuvant / additive

 

 

Carriers (KLH) MaxiBind TiterMax Hybridokine

 

Æ

 Reliable and efficient alternative to the Freund’s adjuvant !
Æ No more batch variations, no more variable and difficult emulsions, no more difficult injections or severe inflammations on your rabbits …
Æ Titers often twice superior with one injection.

  • Non toxic

Free of any proteins, peptides or bacterial/vegetal compounds

  • Stable emulsion 

Emulsifiers and stabilizers are chosen for a better ratio between efficiency and toxicity

  • Efficient

High titers observed within 12 weeks without boost, thanks to proprietary immunomodulators

  • Reliable 

Synthetic and reproducible product, unique formulation

 

How to order

Titer MaxTM classic

R-1

0,5ml

TiterMaxTM gold

G-1

1ml

Technical Sheet

5x0,5ml

Technical Sheet

5x1ml

Both classic TiterMaxTM and Gold TiterMaxTM suit many applications.

The one or the other is sometimes prefered because of particular performances according to the nature of the antigen or the immunization method.

 

Comparison between TiterMaxTM / Complete Freund Adjuvant
-

TiterMaxTM adjuvants

Complet Freund adjuvants

Vehicule

Squalene

Mineral oil

Adjuvant

Modern Copolymere

Tuberculosis mycobacterium

Emulsifier

Non-ionic tensio-active

Non-ionic tensio-active

Stabilizer

Microparticules covered with copolymeres

None

Emulsion formed

Water in oil

Water in oil

Security

Transitory inflammation

Severe transitory inflammation

Efficiency 

The TiterMax™ without boost often exceeds the Freund with boost

Powerful adjuvant

Manufacturer

Strictly controlled manufacturing process

Variable quality according to manufacturers

Preparation

Similar procedure to Freund, creates a more stable emulsion,

injectable with a needle

Little stable emulsion

Difficult to inject
* Classic TiterMaxTM contains copolymere CRL-8941 ; TiterMaxTM Gold contains copolymere CRL-8300

 

Suggested dosage with TiterMaxTM Classic (R-1)

Suggested dosage with TiterMaxTM Gold (G-1)

Species

Method

of

injection

Number

of

injection

Volume

of

injection

Injection place

Species

Method

of

injection

Number

of

injection

Volume

of

injection

Injection place

Mouse

IM

2

20µl

Each quadricep

Mouse

IM

1

100µl

Each quadricep

-

SC

1

40µl

Base of the tail

-

SC

1

100µl

Base of the tail

Rat

IM

2

50µl

Each quadricep

Rat

IM

2

100µl

Each quadricep

Guinea-pig

IM

2

50µl

On each shoulder and each quadricep

Guinea-pig

IM

2

100µl

Each quadricep

-

SC

4

50µl

Each quadricep

-

SC

4

100µl

On each shoulders and each quadricep

Rabbit

IM

2

40µl

Each quadricep

Rabbit

IM

2

100µl

Each quadricep

-

SC

4

100µl

On each shoulder and each quadricep

-

SC

4

200µl

On each shoulders and each quadricep

-

ID

10

10µl

Along the neck

Chicken/Turkey

IM

2

100µl

In each pectoral muscle

Goat/Sheep

IM

4

250µl

2 injections in each quadricep

-

SC

1

200µl

In the neck

Chicken/Turkey

IM

2

50µl

In each pectoral muscle

Cat/Dog

IM

2

200µl

Each quadricep

-

SC

1

100µl

In the neck

-

SC

1

200µl

Along the neck

Horse/Cow

SC

10

100µl

Along the neck

Monkey

IM

2

200µl

Each quadricep

Some good responses were obtained with a relation water/TiterMax ranging from 10/90 to 90/10 but the relation 50/50 is usually optimum.

Rhesus

SC

4

100µl

Inner thigh

Goat/Sheep

IM

4

400µl

2 injections in each quadricep

IM : Intramuscular

Horse/Cow

SC

10

200µl

Along the neck

SC : Subcutaneous

Some good responses were obtained with relation water / TiterMax™ of 50/50.

BACK

 

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