Uptibeads

Separation Techniques

UptiBeads - Magnetic Beads

Polystyrene Microspheres UptiBeads

Polystyrene microspheres UptiBeads are the most judicious choice of magnetic beads for your usual applications. The excellent rate capture of our super para-magnetic beads are obtained thanks to a very advantageous diameter/ligand load ratio, with a very low dispersal rate size beads. UptiBeads are stable between pH2 and 12, compatible with sonication. They are supplied at 1% suspension in PBS, ready-to use.

Catalog-available beads diameters are 0.3 and 1.0µm, and graffted ligands are high affinity anti IgG (all isotypes), anti IgM, Streptavidin and anti Biotin. Ask Uptima for other bead sizes, conditionning, or other ligand of your interest !

High binding capacity
Monodisperse
Excellent colloidal stability
Stable
The most advantageous specific surface/magnetic capacity

Applications:

Cell Biology:

Depletion of cells (negative selection)
Isolation of cells and bacteria (positive selection)
Concentration of cells and bacteria
Lateral flow tests (visualization of cell moves)

Diagnostic:

Immuno-assays (enzymatic, colorimetric, or chemiluminescent detection) (in tube, in microplates)
Agglutination tests (on slides…)
Immunoanalysis

Molecular Biology:

Purification of Igs (i.e. immune IgGs)
Immuno-precipitation
Immuno-capture
Purification (proteins, complexes, receptors…)
Isolation of mRNA et DNA
Hydridization of nucleic acids

Diameter	% ferrite	Density	Particular surface	Number of beads
(µm)	-	(g/cm³)	(area (cm²)	(nb/ml)
0.31	40%	1.54	3.0 x 10^-9	4.2 x 10¹¹
1.0	55%	1.54	3.1 x 10^-8	1.6 x 10¹⁰