DIALYSIS / DESALTING

SPECTRA/POR MEMBRANES 

 

 

Spectra/Por is the most trusted and used name in laboratory dialysis. These membranes exist:

  • in regenerated cellulose (RC), made of cellulose linters (Spectra/Por 1.2.3.4), or natural cellulose and paper (Spectra/Por 5.6.7), with the high quality grade for Biotech RC membranes. See also CelluSep.

  • in cellulose acetate (CE), made from single compounds of pure cellulose, with the high quality grade for Biotech CE membranes.

 

Quick Selection Guide for Spectra/Por Tubings

Technical Information on Membranes

Overview of Spectra/Por Membranes

 

Quick Selection Guide for Spectra/Por Tubings

Classic applications of dialysis

RC membranes as first intention

molecules >10kDa

SpectraPor 1 (SpectraPor 4 / lower pore density and dialysis kinetic)

molecules >20kDa

SpectraPor 2 (SpectraPor 5 / reinforced membranes)

molecules ca3.5kDa

SpectraPor 3

Specific applications / requirements

CE membranes or specific tubings

convenience of use

SpectraPor 6 / provided wet and ready to use, DispoDialysers, SpectraPor 1.2.3.4 ready-to-use dialysis sacks.

cristalography studies

SpectraPor 7 / minimal disulfide and metal traces, Metal and Sulfide removal solutions, Biotech CE and Biotech RC membranes

organic solvant containing buffers

SpectraPor F

-

See also Small Volume Sample Dialysis

 

Technical Information on Membranes

Spectra/Por membranes are obtained:

  • by traditionnal manufacturing, for standard quality

  • by a process that eliminates the use of metal salts in manufacturing (Biotech RC membranes, Biotech CE membranes). No special precleaning is required, thanks to exceptional freedom from impurities such as sulfide and metal contaminants which are commonly found in standard dialysis membranes.

Sterilized membranes are obtained by irradiation (available in Biotech RC and Biotech CE membranes)

SpectraPor membranes material are made of:

  • regenerated cellulose (RC) made from cellulose linters (Spectra/Por 1.2.3.4), or natural cellulose and paper (Spectra/Por 5.6.7), with the high quality grade for Biotech RC membranes

    RC membranes may be chosen in first intention. It is best RC membranes be cleaned before use, except with the Biotech grade.

  • cellulose acetate (CE), made from single compounds of pure cellulose, with the high quality grade for Biotech CE membranes.

    CE membranes don't have to be cleaned before use. CE membranes are sensitive to organic solvents. Strong polar solvents such as acetone, MEK or dioxane will irreparable damage the membrane. This membrane will tolerate exposure to lower alcohols (methanol, ethanol, and isopropanol) with out apparent damage, but with possible alteration of MWCO. The membrane should not be exposed to organic solvents at concentrations exceeding 5% in water. CE membranes exhibit hydrophilic and uniform molecular porous dialysis membranes.

  • in PVDF (Spectra/Por Type F). PVDF membranes have a wide compatibility with various aquous acids and bases and organic solvants, or resistantce to heat. But protein adsorbtion is higher.

Overview of Spectra/Por Membranes

Membrane Type

Material

MWCO

pH Range

Temperature Limit

Organic Solvent Resistance

Available Irradiated

Dialysis Rate

Spectra/Por 1 membranes are recommanded for classic uses for biomolecules above 10kD (MWCO 6-8Kda).

1

RC

6k to 8k

2 to 12

60°C

Many OK

No

High

Spectra/Por 2 membranes are recommanded for classic uses for proteins above 20Kda (MWCO 14-18Kda) or for quicker dialysis kinetics.

2

RC

12k to 14k

2 to 12

60°C

Many OK

No

High

Spectra/Por 3 membranes are recommanded for small biomolecules (MWCO 3.5Kda).

3

RC

3.5k

2 to 12

60°C

Many OK

No

High

Spectra/Por 4 membranes are similar to Spectra/Por 2, but with lower pore density and dialysis kinetic.

4

RC

12k to 14k

2 to 12

60°C

Many OK

No

High

Spectra/Por 5 membranes are reinforced membrane to withstand high pressure differentials, but but dialyzes slower than either Spectra/Por 2 or Spectra/Por 4.

5

RC

12k to 14k

2 to 12

60°C

Many OK

No

Moderate

Spectra/Por 6 membranes are recommanded when convenience of use, as they are in a variety of MWCOs from 1 to 50KDa.

6

RC

1k to 50k

2 to 12

60°C

Many OK

No

High

Spectra/Por 7 membranes are recommanded for applications where impurities (metal, disulfides) may be crucial (i.e. before MS analysis, for enzyme activities, cristalizations…). Biotech membranes are an alternative choice.

7

RC

1k to 50k

2 to 12

60°C

Many OK

No

High

Biotech RC and Biotech CE membranes are the most pure membranes available today. They are produced with sharp, rigidly controlled MWCOs, and provide wet and ready-to-use.

Biotech CE

CE

100k to 300k

2 to 9

37°C

No

Yes

High

Biotech RC

RC

3.5k to 60k

2 to 12

60°C

Many OK

Yes

Highest

Spectra/Por® Type F Dialysis Membranes are recommanded when a wide compatibility is desired with various aquous acids and bases and organic solvants, or resistantce to heat. But protein adsorbtion is higher.

Type F

PVDF

80k to 2.000k

1 to 14

130°C

Most OK

No

Highest

Sterilized membranes are available in Biotech CE, and Biotech RC membranes.

See also DispoDialysers (CE, RC), FloatAlyser, MicroDispoDialysers, CelluSep.

 

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