HPLC

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Uptisphere Applications

Bioanalysis

Uptisphere 120 Å 3µ C18-ODB, 30 x 2.0 mm Pure product

Uptisphere 120 Å 3µ C18-ODB, 30 x 2.0 mm Limit of Quantification

Uptisphere 120 Å 3µ C18-ODB, 30 x 2.0 mm

Extract : 500 µl of plasma after SPE C18 extraction

Malondialdehyde

Uptisphere 3µ BioP 2, 100 x 2.0 mm

Anti-paludeen drugs

Uptisphere 120 Å 5µ C18-ODB, 250 x 4.6 mm

Anti-inflammatory drug

Uptisphere 120 Å 5µ C18-ODB, 250 x 4.6 mm

Syrup (Pharmaceutical + conservatives, without sugar)

Uptisphere 120 Å 5µ C18-ODB, 250 x 4.6 mm

Bioanalysis

Uptisphere 120 Å 3µ C18-ODB, 30 x 2.0 mm

Pure Product

Injection 30µl

266>152 4ng ISTD

278>152 0.04ng

Dectection :

MS/MS Electrospray

+ CID Argon. Micromass QuattroLCZ

UP3ODB#3MS

Uptisphere 120 Å 3µ C18-ODB, 30 x 2.0 mm

Limit of Quantification

Injection quantity

278>152 5pg

266>152 4ng

UP3ODB#3MS

Uptisphere 120 Å 3µ C18-ODB, 30 x 2.0 mm

Extract : 500 µl of plasma after SPE C18 extraction

Injection 30µl

266>152 4ng ISTD

278>152 0.04ng

Dectection :MS/MS Electrospray + CID Argon. Micromass QuattroLCZ

UP3ODB#3MS

Flow rate : 0.150ml/min

A : H2O + 0.1% Formic acid - B : Acetonitrile + 0.1% Formic acid

Gradient : 10% B in 1.5 min ; 3 min a 10% B ; 0% phase B en 0.5 min

2 min stabilisation - Run time : 6 min

Courtesy of Dpt Bioanalyse/PRI/MAP Bristol-Myers Squibb Saint-Nazaire France

Malondialdehyde

Anti-paludeen drugs

Uptisphere 3µ BioP 2, 100 x 2.0 mm

1- Malondialdehyde

2- IS Risperidone

UP3BP2#10MS

NH4 15mM, pH : 2
11 % Methanol

11 % ACN
Flow rate : 190 µl /min
Temp : 55°C - Detection : MS

1- Didesethylchloroquine

2- Monodesethylchloroquine

3- Chloroquine

4- Chlorophenylquanil

5- Cycloquanyl

6- Roquanil 

UP5ODB-25K 

ACN 28%

MeOH 20% - HClO4 30 mM - (pH : 4) 52% - 0.6 ml/min

Anti-inflammatory drug

Uptisphere 120 Å 5µ C18-ODB, 240 x 4.6 mm

23.29 Drug

UP5ODB-25K

ACN 500 ml

H2O 500 ml

H3PO4 1 ml

0,8 ml/min

Syrup (Pharmaceutical + conservatives, without sugar)

Uptisphere 120 Å 5µ C18-ODB, 250 x 4.6 mm

83 % KH2PO4 0.05 M including
15 % heptane sulfonate Na

17 % ACN

Flow : 1.5 ml/min

UV : 280 nm

UP5ODB-25QS

211 Bis Avenue J.F. Kennedy - BP 1140 - 03103 Montluçon cedex - France

Tel. (33)(0)4 70 03 88 55  - Fax (33)(0)4 70 03 82 60 - Hot line: (33)(0)4 70 03 73 09

interchrom@interchim.com - www.interchim.com

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