HPLC

BACK To Uptisphere Application Selection Guide

Uptisphere Applications

Basic Drugs

Uptisphere 120 Å 5µ C18-ODB, 250 x 4.6 mm

Kawalactones from piper methysticum FORST

Uptisphere 120 Å 5 µ ODB, 250 x 3.9 mm

Cotinine (urinary pool std 100µ/l)

Uptisphere 120 Å 5µ C18-ODB, 250 x 4.6 mm

Urine extract (cotinine 52 µ/l)

Uptisphere 120 Å 5µ ODB, 250 x 4.6 mm

Flavors & Fragrances

Uptisphere 120 Å 5 µ HDO, 150 x 3.9 mm

N-Methylcarbamates in water

Uptisphere 120 Å 5µ C18-HDO, 150 x 4.6 mm

Amines oxides

Uptisphere 120 Å 5µ C18-ODB, 250 x 4.6 mm

Herbicides

Uptisphere 120 Å 5µ ODB, 250 x 4.6 mm

Basic Drugs

Kawalactones from piper methysticum FORST

Uptisphere 120 Å 5µ C18-ODB, 250 x 4.6 mm

Uptisphere 120 Å 5µ ODB, 250 x 3.9 mm

5.03 Nicotine

6.17 Tranhydroxycotinin

10.84 Cotinin

16.38 Caffeine

UP5ODB-25QS

27% MeOH

73% Acetate buffer pH : 5.73

Flow rate : 1 ml/min

12.85 Desmethoxyyangonine

16.55 Dihydrokawaine

19.50 Yangonine

23.06 Kawaine

26.91 Dihydromethysticine

38.03 Methysticine

UP5*25K 

Hexane

Dioxane

1.5 ml/min

UV 240 nm

(Courtesy of Pr Ch Bartomeuf phD Clermont-FD University)

Cotinine (urinary pool std 100µ/l)

Urine extract (cotinine 52 µ/l)

Uptisphere 120 Å 5µ C18-ODB, 250 x 4.6 mm

Uptisphere 120 Å 5µ ODB, 250 x 4.6 mm

UP5ODB-25QS

ACN 10% - Buffer (pH : 4.4) 90%

1.5 ml/min - 45°C - UV 262 nm

UP5ODB-25QS

ACN 10% - Buffer (pH : 4.4) 90%

1.5 ml/min - 45°C - UV 262 nm

Flavors & Fragrances

Uptisphere 120 Å 5µ HDO, 150 x 3.9 mm

5.63 5-MeFurfural OH

15.09 Me-5-Furfural

16.95 Vanilic acid

21.05 Syringic acid

UP5HDO*15QS

UV 295 nm

N-Methylcarbamates in water

Amines oxides

Uptisphere 120 Å 5µ C18-HDO, 150 x 4.6 mm

Uptisphere 120 Å 5µ C18-ODB, 250 x 4.6 mm

4.84 Aldicarb sulfoxide

5.62 Aldicarb sulfon

5.92 Oxamyl

7.30 Methomyl

14.60 Carbofuran 3-hydroxide

20.86 Aldicarb

28.24 Propoxur

29.09 Carbofuran

32.30 Carbaryl

45.70 Methiocarb

46.57 Internal std BDMC

UP5HDO-15K

H2O-MeOH (t = 0 (80/20) ;

t = 40 (45/55) ;

t = 50 (0/100) during 5 min)

1 ml/min

OPA derivatization : ex 330 nm ;

em 466 nm

UP5ODB-25K

MeOH 72% - ACN 9%

H2O 19%

1 ml/min

40°C

Herbicides

Uptisphere 120 Å 5µ ODB, 250 x 4.6 mm

UV 220 nm

21.25 Mevinphos

28.80 Dicamba

31.06 Bromoxynil

31.44 2.4-D

34.46 MCPA

34.90 2.4.5-T

44.16 Coumaphos

UV 240 nm

29.31 Chlorotoluron

30.50 Isoproturon

30.95 Diuron

34.22 Ioxynil

35.97 Linuron

UP5ODB-25QS

UV 270 nm

39.17 Diflubenzuron

43.64 Dinoterbe

211 Bis Avenue J.F. Kennedy - BP 1140 - 03103 Montluçon cedex - France

Tel. (33)(0)4 70 03 88 55  - Fax (33)(0)4 70 03 82 60 - Hot line: (33)(0)4 70 03 73 09

interchrom@interchim.com - www.interchim.com

BACK