HPLC

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BioChromatography Applications

Octapeptide

Competitor

Uptisphere 120 Å 5µ C18-ODB, 250 x 4.6 mm

Trypsin digest of Monoclonal IgG

Uptisphere 300 Å 5µ C18-ODB, 250 x 3.9 mm

BSA : 20 pmoles on 1 mm id column

-

OnLine IgG peptide mapping and differential display of glycopeptides

Octapeptide

Competitor

Same dimensions of column. The main peak could be eluted faster by increasing the strength of the eluant.

Trypsin digest of Monoclonal IgG

BSA : 20 pmoles on 1 mm id column

Uptisphere 300 Å 5µ C18-ODB, 250 x 3.9 mm

-

A : TFA 0.115%

B : MeCN + TFA 0,1%

Flow rate : 0.5 ml/min

0-5% : 0.1 cv ; 45% : 20 cv ; 80% : 3 cv

UP5WOD$25QS

Courtesy of LFB

TFA, A : 110 µl —> B : 100 µl

inj at 20 min

UV 214 nm

60 µl/min

UPODBD15

BSA

20 pmoles Amidoblack digest by 0.4 µg of Endok in 200 µl of Tris buffer pH : 8.6 + SDS 0.03%

coupled with DEAE column

OnLine IgG peptide mapping and differential display of glycopeptides

A: H2O-IFA 100/0.115% v/v

B: ACN-H2O-TFA 90/10/0.1% v/v

column is equilibrated with digestion

buffer 12% to 70% B in 110 min

0,5 ml/min ; UV 214 nm

UP5ODBD-25K

Courtesy of LFB

211 Bis Avenue J.F. Kennedy - BP 1140 - 03103 Montluçon cedex - France

Tel. (33)(0)4 70 03 88 55  - Fax (33)(0)4 70 03 82 60 - Hot line: (33)(0)4 70 03 73 09

interchrom@interchim.com - www.interchim.com

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