HPLC

 

Uptisphere, Supporting Biochromatography Applications

 

 

Proteins, peptides and other large biomolecules are generally not suited to separation using to supports based upon 120 Å silica. 300 and 500 Å silica have been successfully utilised to support many purification requirements for biomolecules.

Uptisiphere 300 and 500 Å silica is manufactured to the same stringent protocols as the smaller particles and provide an excellent support for the purification of biomolecules.

Advantages

  • High efficiency

  • Ultrapure silica

  • pH stability : between 2 to 7.5

  • Perfect reproducibility

Results of the efficiency tests :

5 µm Efficiency > 80 000 plates/m
10 µm Efficiency > 40 000 plates/m
15 µm Efficiency > 25 000 plates/m
20 µm Efficiency > 15 000 plates/m

Silica purity > 99.995 % guarantees a minimum of non specific interactions.

Biochromatography Uptisphere® silicas are totally compatible with ordinary mobile phases for the separation of biomolecules.

Their pH is very stable, especially in an acidic environment.

WOD, WRP, WC8 and WC4 uptisphere® phases are available in 15 and 20 µm for purification.

Interchim's Upti-trap provides an excellent means of sample preparation and facilitates the optimisation of subsequent sample analysis.

E.g.

  • SDS removal

  • Peptide/protein concentration

  • Sample desalting

 

Selectivity Guide

Bonding

WOD

WRP

WC8

WC4

WTF

 WT4 XTF

-

Pore size

300A

Surface area

100 m2/g

Bonding technology

 Monofunctional Polyfunctional Trifunctional

Carbon Content

10%

9%

8%

4%

8%

 3% na

End-capping

yes

na

yes

yes

yes

 yes yes

-

3µm

x

-

-

x

-

-

-

5µm

x

x

x

x

x

x

x

10µm

x

x

x

x

-

-

-

15µm

x

x

x

x

-

-

-

20µm

x

-

-

x

-

-

-

Soon Available

 

ATOLL

Atoll is a family of spherical macro-porous co-polymers which are extremely pure and very higly cross-linked. Atoll is extremely pure and has been designed to support applications such as solid phase sample preparation and HPLC analysis of biomolecules.

  • Ultra pure

  • High mechanical resistance
  • Pharmaceutical grade

Rigorous manufacturing protocols, combined with a judicious choice of monomer ratio, guarantees Atoll's excellent mechanical stability. The Atoll polymeric support does not "swell" and can be used with all ordinary solvents.

  • pH range : 0 - 14

Atoll polymeric supports do not suffer from irreversible absorption (a problem inherent with traditional silica based supports). Sample bio-activity is therefore maintained and sample output optimised an essential feature of this purification tool.

Atoll NPR :

non-porous resin with a 2 µm granulometry dedicated to ultra-fast analyses of biomolecules in LC/MS, MS/MS…

Atoll MP : 

porous resin recommended for the purification of pharmaceutical molecules, small basic molecules and peptides

Atoll MP3 : 

macro-porous resin adapted to the separation of hydrophyl peptides, proteins, recombinant proteins and bio-purification.

 

Selectivity Guide

Bonding

Atoll NPR

Atoll MP

Atoll MP3

-

Pore size

0

 n.a. 300A

Surface area

2.5 m2/g

 500 m2/g 500 m2/g

Bonding technology

 non-functional

-

2µm

x

-

-

3µm

-

on request

-

5µm

 - x x

10µm

-

on request

-

15µm

-

x

x

30µm

-

on request

-

 

Proteins mixture

Atoll 5 µ MP, 50 x 4.6 mm

 

Mobile phase :

H2O + 0.1% TFA to ACN
+ 0.1% TFA (15min)

Flow rate : 1 ml/min

Detection : UV 254 nm

 

 

1. Ribonuclease A

2. Cytochrome C

3. Holo-Transferrine

4. Apomyoglobine

Atoll polymers are available in several sizes and harwares (depending on the application).

  • Columns, cartridges and 96 well plates for sample preparation and purification

  • Nano / Micro columns
  • Modulo-cart Quick seal
  • Modulo-cart prep
  • Easy-pack
  • Low pressure glass column

  • Columns and cartridges for flash chromatography

 

211 Bis Avenue J.F. Kennedy - BP 1140 - 03103 Montluçon cedex - France

Tel. (33)(0)4 70 03 88 55  - Fax (33)(0)4 70 03 82 60 - Hot line: (33)(0)4 70 03 73 09

interchrom@interchim.com - www.interchim.com

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