HPLC

 

 

Uptisphere, Speciality Columns

 

 

Uptisphere, Mixed-Mode

Uptisphere Mixed-mode phases are characterized by their double functionality.

  • RCX and MM1 : Hydrophobic / Strong Cation Exchanger (SCX)

  • RAX : Hydrophobic / Strong Anion Exchanger (SAX)

  • RPH : Hydrophobic / Phenyl

Uptisphere RCX, RAX and RPH has a bi-functional silane.

Uptisphere MM1 has two different silanes bonded on the silica.

Compared to traditional phases, the Uptisphere Mixed-mode fashion permits a better discrimination of the molecules of a complex mixture.

 

Applications:

  • RCX : analysis of basic polar and non polar compounds

  • RAX : analysis of acidic polar and non polar compounds

  • RPH : analysis of non polar compounds, molecules with plan effect or electronic delocalisation

  • MM1 : Drugs of abuse

 

pH stability

Temperature stability

2.75 < RCX - RAX < 7.5

Max : 50 °C

2 < MM1 - RPH < 7.5

 

µm

mm

RPH

RAX

 RCX

MM1

2.0 mm I.D.

5

150 x 2.0

UP5RPH#15QS

UP5RAX#15QS

UP5RCX#15QS

UP5MM1#15QS

250 x 2.0

UP5RPH#25QS

UP5RAX#25QS

UP5RCX#25QS

UP5MM1#25QS

3.9 mm I.D.

5

150 x 3.9

UP5RPH*15QS

UP5RAX*15QS

UP5RCX*15QS

UP5MM1*15QS

250 x 3.9

UP5RPH*25QS

UP5RAX*25QS

UP5RCX*25QS

UP5MM1*25QS

4.6 mm I.D.

5

150 x 4.6

UP5RPH-15QS

UP5RAX-15QS

UP5RCX-15QS

UP5MM1-15QS

-

250 x 4.6

UP5RPH-25QS

UP5RAX-25QS

UP5RCX-25QS

UP5MM1-25QS

 

µm

mm

RPH

RAX

 RCX

MM1

2.0 mm I.D.

5

150 x 2.0

UP5RPH#15QK

UP5RAX#15QK

UP5RCX#15QK

UP5MM1#15QK

250 x 2.0

UP5RPH#25QK

UP5RAX#25QK

UP5RCX#25QK

UP5MM1#25QK

3.9 mm I.D.

5

150 x 3.9

UP5RPH*15QK

UP5RAX*15QK

UP5RCX*15QK

UP5MM1*15QK

250 x 3.9

UP5RPH*25QK

UP5RAX*25QK

UP5RCX*25QK

UP5MM1*25QK

4.6 mm I.D.

5

150 x 4.6

UP5RPH-15QK

UP5RAX-15QK

UP5RCX-15QK

UP5MM1-15QK

-

250 x 4.6

UP5RPH-25QK

UP5RAX-25QK

UP5RCX-25QK

UP5MM1-25QK

 

 

Uptipshere 5 RCX

Uptipshere 5 RAX

150 x 4.6 mm

 

 

1. Benzyltriethylammonium

2. Benzyltrimethylammonium

150 x 4.6 mm

 

CH3CN/H2O/TEA/AcOH = 60/40/1/1

1.0 ml/min

UV254 nm

 

1. Benzensulfonamide

2. Benzensulfonic acid

Comparative

                UP5RAX                            

C18 Standard

SAX Standard

Mobile Phase :

0.005M KH2PO4

(pH 3.0)/MeOH = 80/20

Detection : UV 254 nm

Temperature : 25°C

Flow rate :1 ml/min

 

 

1. 5'-Inosinic acid

2. 5'-Adenylic acid

3. 5'-Thymidylic acid

 

Uptisphere, DNAP

This silica has been originally produced in our laboratories upon request of the "Institut Français du Pétrole".

5 and 40 µm particle size are respectively available for analytical and sample preparation.

 

Application :

Separation of aromatic compounds by number of cycles.

Arjuna (terrestrial o.m)

Boscan (marine o.m.)

Conditions

UV detection 254 nm

0-11 min : hexane 0.5 ml/min

11-15 min : hexane 1 ml/min

15-25 min : 1 ml/min gradient hexane / DCM 80/20

25-35 min : 1 ml/min gradient DCM 100

Retention time

7-10 min : monoaromatics

10-18 min : C3/C4 naphthalenes

18-25 min : C1 fluorenes / dibenzothiophenes

25-28 min : phenanthrene

(GC/MS identification)

Courtesy of Mme Vandenbroucke sce Géochimie IFP

 

Standard mixtures separation on DNAP

and TCP columns : mono- and di-aromatics

µm

mm

DNAP

2.1 mm I.D.

5

250 x 2.1

UP5DNAP#25QS

4.6 mm I.D.

5

250 x 4.6

UP5DNAP-25QS

40

250 x 4.6

UP40DNAP-25QS

10.0 mm I.D.

5

250 x 10.0

UP5DNAP.25M

40

250 x 10.0

UP40DNAP.25M

21.2 mm I.D.

5

250 x 21.2

UP5DNAP/25M

40

250 x 21.2

UP40DNAP/25M

 

211 Bis Avenue J.F. Kennedy - BP 1140 - 03103 Montluçon cedex - France

Tel. (33)(0)4 70 03 88 55  - Fax (33)(0)4 70 03 82 60 - Hot line: (33)(0)4 70 03 73 09

interchrom@interchim.com - www.interchim.com

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