SPE

 

 

Introduction

Sample preparation is arguably the most critical step within the analytical process. Interchim have placed considerable time and energy into our product development programmes to assist clients with this aspect of the analysis process and considerable improvements have been made over the last five years.

Sudies have reported that sample preparation can represent up to 60 % of a laboratory technicians timetable and also forms one of the principal sources of error. Sample preparation undoubtedly has a fundamental impact on the subsequent quality of analysis affecting a wide range of analytical parameters.

A variety of proven techniques, such as filtration, dialysis, liquid/liquid extraction and Solid Phase Extraction (SPE), are routinely adopted in today's analytical laboratoires to resolve the vast array of sample preparation demands.

SPE has estabilished itself as one of the most popular and flexible tools within the analytical laboratory ; providing effective, efficient sample concentration and purification prior to HPLC, LC/MS, GC or GC/MS and adaptation to automated techniques such as combinatory chemistry.

Interchim's laboratories have developed a range of SPE products from their core analytical supports i.e. Silica (Upti-prep) and Polytyrene divinylbenzene (Atoll).

The resultant SPE product range provides unparalleled performances and satisfies the clients demand for Recovery, Reliability and Reproducibility.

 

General Methodology

SPE methods consist of several steps:

  • Conditionning

    Condition the absorbent with an organic solvent such as MeOH or a solvent mix to eliminate contaminants and activate the media. The absorbent should not be allowed to dry before adding the sample.

  • Sample introduction

    Into the top part of the absorbent bed and either push or pull the sample through the bed at a low flow rate.

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  • Wash

    (Only necessary if your compounds of interest has been absorbed onto the bed). Rince to remove all interfering agents with a solvent or suitable solvent mixture (0 % affinity for the compound of interest 100 % for interfering agent. A number of washing phases with different solvents may be required. This being usually dependent upon the nature of interfering substances.

    •

  • Elution

    The absorbed sample must be eluted with a solvent or a solvent mixture that has 100 % affinity for the sample and 0 affinity for any potential interfering agents.

    •

  • Dry

    (If necessary), elute with anhydrous sodium sulfate to remove all traces of water

  • Concentration

    (If necessary), concentrate the compound of interest by an evaporating step and retake it with the analysis mobile phase before injection for analysis.

 

Containers

Interchim offer a wide selection of SPE container formats.

  • Polypropylene tanks (syringe type) - medical grade

    Volumes available : 1-3-6-15-25-75-150ml

    Frits : 10-20-70µm/PE/PTFE

  • Large polypropylene tanks (LRC)

    Volume : 15ml

    Frits : 20µm PE

  • Glass tanks

    Volume available : 1-3-6-20ml

    Frits : 20µm PTFE-glass fiber

  • Polypropylene cartridge-medical grade

    Absorbent mass : 300-600-900mg

    Frits : 20µm PE

  • 96 wells plates

    For development: 1 to 2 ml square individual well/20µm PE frits

    For routine: 500µl round well/10µm PE frits; 2ml square well/20µm PE frits

[Precision bed weight ± 1 %]

Custom manufacture enquires are welcome.

 

211 Bis Avenue J.F. Kennedy - BP 1140 - 03103 Montluçon cedex - France

Tel. (33)(0)4 70 03 88 55  - Fax (33)(0)4 70 03 82 60 - Hot line: (33)(0)4 70 03 73 09

interchrom@interchim.com - www.interchim.com

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