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SUPPLIERS > Tosoh Bioscience > TSKgel HPLC Columns
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Tosoh Bioscience GmbH

Tosoh’s product portfolio encompasses a comprehensive line of TOYOPEAR®L
and TSKgel® chromatography media and prepacked TSKgel® HPLC columns.

Tosoh Bioscience offers chromatography solutions for research, drug discovery, medicinal chemistry, environmental analysis, manufacturing and other industrial applications for all common modes of liquid chromatography, including IEC, HIC,
RPC, HILIC, SEC and affinity.

Read our Chromatography Catalog on TSKgel® columns and TOYOPEARL® media here!

 

 

TSKgel® HPLC columns
Tosoh Bioscience offers a broad range of prepacked columns for high-performance liquid chromatography (HPLC). They include the most widely used modes of liquid chromatography, such as ion exchange, hydrophobic and hydrophilic interaction, reversed phase, size exclusion, and affinity.

TSKgel® columns for high-performance liquid chromatography are renowned for their quality and reliability. Filled with high purity silica or polymer based resins and functionalized with a broad range of ligands the columns are utilized in a variety of research and day-to-day applications. Over 500 scientific publications, using TSKgel® columns, prove their outstanding quality and cutting-edge technology. 
For over thirty years Tosoh has continued to be the world leader in the analysis of proteins—our silica based TSKgel® SW columns are the industry standard for size exclusion chromatography (SEC) of proteins. The latest additions to the column portfolio comprise HILIC and reversed phase columns, filled with sub 3 µm particles—ideally suited for fast, high resolution and high sensitivity LC-MS analysis, as well as new SEC columns for improved antibody analysis.
 
Featured product
TSKgel® mAb SEC columns tailored for various aspects of antibody analysis

TSKgel® G3000SWXL columns have been the industry’s standard for quality control of monoclonals by SEC for decades. Based on the proven proprietary surface technology of the renowned TSKgel® SW series, a new series of silica-based SEC columns was engineered to provide shorter analysis time or higher resolution for antibody analysis.
 
HIGHLIGHTS
  • Optimized for antibody analysis
  • Small particle size for UHPLC use
  • Highest resolution with SuperSW mAb HR
  • Fast separation with SuperSW mAb HTP
  • Higher molecular weight range with UltraSW Aggregate
 
More information on the new TSKgel® SEC columns.
 

 

TOYOPEARL® and TSKgel® chromatography resins

TOYOPEARL® chromatography resins are hydrophilic, macroporous, bulk processing media designed especially for large-scale chromatography applications. TOYOPEAR®L resins are available for the most common modes of liquid chromatography: size exclusion (SEC), hydrophobic interaction (HIC), ion exchange (IEC) and Affinity chromatography (AFC).

Based on their semi-rigid backbone structure TOYOPEARL® resins assure excellent pressure/flow characteristics. TOYOPEARL® is stable over the pH 2-13 range. Particle sizes are 20-40 µm superfine grade (S) for the highest performance, 40-90 µm for economical purification (M), and 90-120 µm coarse grade (C) and 100 - 300 µm extra coarse grade (EC) for the largest scale chromatography.

Similar Chemistries of TOYOPEARL® and TSKgel® columns allow seamless scale up. TOYOPEARL® polymeric resins are available as bulk resin and in prepacked ToyoScreen® columns for convenient screening.
 
Featured product
TOYOPEARL® AF-rProtein A HC-650F the ultra-high capacity Protein A resin

The new TOYOPEARL® AF-rProtein A HC-650F is designed for high-throughput processing of high titer feedstock. It achieves 30% to 50% greater antibody adsorption than similar products and can considerably improve process economics.
 
HIGHLIGHTS
  • Superior dynamic binding capacity for human IgG (> 70 g/L)
  • Capacity increases with feedstock titers up to 10 g/L and higher
  • Alkaline stability through engineered recombinant ligand
  • Minimized leaching through multi-point attachment
The new Protein A resin is available as bulk material, pre-packed ToyoScreen® column and ToyoScreen® RoboColumnTM for automated resin screening
 
More information on the new  TOYOPEARL® AF-rProtein A HC resin.

 


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