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INTERFINE CHEMICALS > Flash Purification - UPFP > puriFlash Columns > puriFlash High Pressure Dry-Load

puriFlash® Dry-load

DryLoad_0A0350_Interchim_0518  Dry-load columns for solid deposits allow the injection of a raw sample insoluble (or soluble) in the mobile phase.
Compared to liquid injection, the solid deposit avoids the diffusion of raw sample in the purification column. It improves the resolution, the efficiency and the purity of the products collected.
The solid deposit that can be made with silica, C18 or Celite. Unlike open cartridges, it does not require the use of a piston or of specific adapters.
The max. pressure is 2x the standard solid deposit cartridges.
They are compatible with the use of Interchim® 15µm puriFlash® columns.
 
  • Luer lockInlet & Outlet
  • 4g to 300g Dry-load
  • Compatible with all flash purification systems

 

Dry-load Injection

The dry-load injection is a convenient technic when the polarity of the reactional mix (or the extract) to purify is close to the polarity of stationary phase or, when it contains solutes with extreme opposite polarity.
It should also be consider when:
  • the compound of interest is a lot more retained than the other compounds we would like to separate.
  • the sample contains one or several compounds having low solubility with the eluent.
Compare to liquid injection, the dry-load injection improves the efficiency, the resolution, and the final purity.

 

Sample in solution

Injection Loop

Injection_Loop_GO2330_Interchim_0518

Limited injection volume
due to sample solubility

 

Solid sample


Dry injection                                                   High pressure dry-load


Solid_Sample_Dry_Injection_Interchim_0518High_Pressure_Dry_Load_Interchim_0518

Allow to large sample amounts

 

How to prepare the Dry-load cell for injection

DryLoad_For_Injection_Interchim_0518


1. Adsorb the dissolved sample in a "better solvent" on a small amount of stationary phase (Silica, C18 or Celite).

2. Evaporate the deposit solvent with a rotary evaporator until a "dry" powder is obtained. (If the volume of the dissolved sample is small, it can be poured onto the silica, and the partially impregnated silica mixed up to obtain a homogeneous dry powder, thus avoiding the passage to the rotary evaporator).

4. Place the mixture over the inlet frit of the column, once it has possibly been equilibrated with the elution solvent.

5. Add a sintered frit over the mixture, then a closure system or the piston of the column (for equipped systems) and tamp the mixture slightly to obtain a perfectly homogeneous deposit thickness.

6. Proceed with elution.

 

Technical tips

The volume of the dry-load must not exeed 5% to 10% of the purification column volume to keep sufficient resolution between fractions.
If possible, wet the dry-load with 100% of the less eluent solvent before to start the purification run.
Caution : Adapt your step in function of the back pressure and the acceptable flow rate.
The dry-load can generate air bubbles creating disruptions that hide the first peaks. (UV detector).

Celite exhibit advantages:
  • It does not generate back pressure due to its large particle size
  • It does not interact with the compounds that arrive at the same time at the top of the column
  • It improves separation and are compatible with both NP and RP mode
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