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LIFE SCIENCES > Immunodetection Tools > COVID-19 RESEARCH > RT-qPCR and RNA extraction kits
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Covid-19: RT-qPCR and RNA extraction kits
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| We offer a range of RT-qPCR kits for testing the new coronavirus strain COVID-19. Our molecular kits are specific in obtaining accurate results, specially in avoiding false-negative outcomes. |
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Total RNA Purification Kit
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For rapid purification of total RNA - including microRNA - without phenol.
- Extract high quality & purity total RNA including miRNA
- No phenol step required
- Bind & elute all RNA irrespective of size or GC content, without bias
- Efficiently extract small RNA irrespective of GC content
- Very sensitive & linear down to a few cells without the need for carrier RNA
- Convenient & fast spin column format
- Isolate from a wide variety of specimens
- Purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing.
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Description |
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Packing |
| 17200 |
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Total RNA Purification Kit |
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50 preps |
| 37500 |
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Total RNA Purification Kit |
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100 preps |
| 17250 |
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Total RNA Purification Kit |
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250 preps |
| 17270 |
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Total RNA Purification Kit |
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500 preps |
| 24350 |
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Total RNA Purification 96-Well Kit (Deep Well Format, height 3.8 cm) |
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2 plates |
| 24380 |
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Total RNA Purification 96-Well Kit (Deep Well Format, height 3.8 cm) |
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6 plates |
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2019-nCoV TaqMan RT-PCR Kit
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| Norgen’s 2019-nCoV TaqMan RT-PCR Kit is designed for the detection of SARS-CoV-2 specific RNA in a real-time RT-PCR based on the use of TaqMan technology. This kit is designed for research use only and not for use in diagnostic procedures. The detection of SARS-CoV-2 specific RNA is based on TaqMan one-step RT-PCR providing a simple, reliable and rapid result for the detection of SARS-CoV-2 infection. Norgen’s 2019-nCoV TaqMan RT-PCR Kit includes a PCR control to monitor for PCR inhibition, and to validate the quality of the sample and the detection result. |
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| The 2019-nCoV TaqMan RT-PCR Kit comprises Master Mix for the target and PCR control detection, 4 target Primer & Probe Mixes, as well as a positive control and a negative control (nuclease-free water) to confirm the integrity of the kit reagen. |
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Detection kit for SARS-CoV-2, which causes COVID-19 (2019-nCoV):
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| Ready to use format containing everything required for detection Available in 1 format for analysis |
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Description |
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Packing |
| TM67100 |
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2019-nCoV TaqMan RT-PCR Kit |
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50 reactions |
| TM67120 |
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2019-nCoV TaqMan RT-PCR Kit |
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500 reactions |
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PCR Detection Solutions for Coronavirus
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For rapid purification of total RNA - including microRNA - without phenol.
- COVID-19 detection based on the assays and protocols developed by the CDC
- Primer & Probe Mixes contain all 3 CDC developed assays in individual tubes
- All assays are premixed to the working concentrations recommended by the CDC
- Positive Control contains 2 nCoV nucleocapsid target gene RNA (N1,N2) and RNase P (internal control) which are compatible with the CDC primer/probe set
- Convenient ready-to-use 2X One-Step RT-PCR Master Mix
- Manufactured under ISO 13485:2016 for research use only
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Description |
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Packing |
| TM67101 |
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2019-nCoV Primer & Probe Mixes |
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50 reactions (per tube) |
| PC67102 |
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2019-nCoV RT-PCR Positive Control |
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50 µL |
| TM67130 |
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2019-nCoV Primer & Probe Mixes |
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500 reactions (per tube) |
| PC67120 |
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2019-nCoV Positive Control |
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500 µL |
| 28113 |
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2X One-Step RT-PCR Master Mix |
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100 reactions |
| 28115 |
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2X One-Step RT-PCR Master Mix |
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500 reactions |
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Fast Plus Evagreen qPCR Master Mix
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| Fast Plus EvaGreen® qPCR master mix is a ready-to-use hot-start mix for nucleic acid quantitation and melt curve analysis of PCR amplicons. Formulated using the environmentally safe EvaGreen® dye and fast-activating chemically-modified hotstart enzyme, Cheetah™ Taq, Biotium´s PCR master mix delivers superior performance even with the most challenging samples tested. |
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EvaGreen® PCR dye is bright and sensitive. Comparison between an EvaGreen® qPCR Master Mix from Biotium and two fast SYBR® Green master mixes from two leading companies (company A and company Q) under similar condition. The inset is an enlarged view of the area near the baseline for better viewing the curve patterns of the weaker signals of the two SYBR®-based master mixes. Amplicon: ATPG fragment of human genomic DNA; instrument: ABI 7900 Fast. |
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| The master mix is offered in three formats containing different amounts of ROX reference dye for use with different PCR instruments (without ROX, low ROX, and high ROX). |
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Description |
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Packing |
| #31014-T |
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FAST PLUS EVAGREEN qPCR MASTER MIX - Low ROX |
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Trial size (1 mL) |
| #31014 |
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FAST PLUS EVAGREEN qPCR MASTER MIX - Low ROX |
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2 x 1 mL |
| #31014-1 |
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FAST PLUS EVAGREEN qPCR MASTER MIX - Low ROX |
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5 x 1 mL |
| #31014-2 |
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FAST PLUS EVAGREEN qPCR MASTER MIX - Low ROX |
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50 x 1 mL |
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ABScript II One Step RT-qPCR Probe Kit (RK20407)
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ABScript II One Step RT-qPCR Probe Kit a ready-to-use kit allowing reverse transcription and subsequent probe-based qPCR in a single tube. It contains all components for RT-qPCR except primers, probes and RNA templates.
The one-step format significantly improves sensitivity and effectively prevent contamination. |
The ABScript II Reverse Transcriptase in the kit provides reliable reverse transcription to a wide range of RNA template amount. After reverse transcription, the Hot-start version of Taq polymerase is activated at 95°C and the ABScript II Reverse Transcriptase is inactivated simultaneously. In the sequential PCR reaction, the 5 ´-3 ´exonuclease activity of Taq polymerase cleaves the hybridized probe, separating the reporter from the quencher and releasing fluorescent signal. The ABScript II One Step RT-qPCR Probe Kit is an ideal product for high-speed analyses of low input RNA sample.
Features : |
- From RNA sample to qPCR data only need one step;
- The core reverse transcripase in this kit is ABclonal´s ABScript II, which could work in various situations. It has advantage of sensitivity and efficiency;
- Suitable for multiplex PCR;
- Could be applied in research, clinical /medical, industrial probe-based RT-qPCR reactions;
- Compatible for all machine types, choosing the ROX types as you wish
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Result Using synthesed sequence from 2019-nCov
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Using the Pseudovirus, synthesed sequence from 2019-nCov, as the template, diluted into 105, 104, 103, 102 and 10 copies. Targets are ORF 1ab gene and N gene (Probes are recommended by CCDC).
The target could be well detected by ABclonal´s kit. |
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Description |
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Packing |
| RK20407 |
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ABScript II One Step RT-qPCR Probe Kit |
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50 reactions |
| RK20407 |
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ABScript II One Step RT-qPCR Probe Kit |
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100 reactions |
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For more information, please see our Covid-19 related article (in french).
In progress. Due to the current situation, this page may be subject to frequent updates.
For any request please contact-us.
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NORGEN BIOTEK 2019-nCoV TaqMan RT-PCR Kit, Detection kit for SARS-CoV-2, which causes COVID-19 P/N : TM67100 Pack : 1 x 50 TS P/N : TM67100 Item : B8D9IA Pack : 1 x 50 TS Cat. Price :  NORGEN BIOTEK COVID-19 TAQMAN RT-PCR KIT (E/RDRP GENES) DX P/N : DXTM67200 Pack : 1 x 500 TS P/N : DXTM67200 Item : BSEHM0 Pack : 1 x 500 TS Cat. Price : NORGEN BIOTEK COVID-19 TaqMan RT-PCR Kit (E/RdRP genes-Charite/Berlin protocol), CE-IVD marked P/N : TM67240 Pack : 1 x 40 TS P/N : TM67240 Item : B8J0CE Pack : 1 x 40 TS Cat. Price : NORGEN BIOTEK COVID-19 TaqMan RT-PCR Kit (E/RdRP genes-Charite/Berlin protocol), CE-IVD marked P/N : TM67200 Pack : 1 x 500 TS P/N : TM67200 Item : B8J0C0 Pack : 1 x 500 TS Cat. Price : NORGEN BIOTEK COVID-19 TAQMAN RT-PCR KIT (N/ORF1AB GENES) DX P/N : DXTM67300 Pack : 1 x 500 TS P/N : DXTM67300 Item : BWIET0 Pack : 1 x 500 TS Cat. Price : NORGEN BIOTEK COVID-19 TAQMAN RT-PCR KIT (N/ORF1AB GENES) P/N : TM67300 Pack : 1 x 500 TS P/N : TM67300 Item : BSEFM1 Pack : 1 x 500 TS Cat. Price : NORGEN BIOTEK COVID-19 TAQMAN RT-PCR KIT (N/ORF1AB GENES) P/N : TM67320 Pack : 1 x 50 TS P/N : TM67320 Item : BSEFM0 Pack : 1 x 50 TS Cat. Price : NORGEN BIOTEK COVID-19/INFLUENZA (A & B) TAQMAN RT-PCR KIT P/N : TM67400 Pack : 1 x 500 TS P/N : TM67400 Item : BSEHL0 Pack : 1 x 500 TS Cat. Price : NORGEN BIOTEK COVID-19/INFLUENZA (A & B) TAQMAN RT-PCR KIT P/N : TM67420 Pack : 1 x 50 TS P/N : TM67420 Item : BSEHL1 Pack : 1 x 50 TS Cat. Price :
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