I am not getting any detection from the UV detector, what should I do?
This can be due to:
- UV cell clogging.
UV cell clogging may be caused by the injected products and solvents used.
Clean the cell with a high-throughput compatible solvent.
If cleaning is ineffective, place the UV cell in a sonicator bath, in a high-eluent solvent or in the presence of H2SO4 or HNO3 diluted ten- or twenty-fold.
If the UV cell is still not clean, then it will need to be replaced.