Why is there no staining in the positive control??
FAQ from Trevigen
1. Verify retention of the agarose sample on the slide. Initial application of agarose sample should cover the entire well on your slide. Once dry there is a 0.5 mm clear ring separating the agarose from the edge of the well, as the agarose will shrink back.
2. Verify that ~1000 cells were present in agarose sample applied to the well.
3. Use fresh aliquot of hydrogen peroxide to create positive control.