Dilution for anti-tubulin sheep polyclonal antibody?
Posted: Wed 29 Jun 2016 10:05At what dilution should I use the anti-tubulin sheep polyclonal antibody?
FAQ from CYTOSKELETON
All dilutions are noted in the datasheet. The actual dilution of antibody depends on the cell/tissue type and experimental format being used. For western blotting, we have tested and recommend using the antibody at a concentration of 500 ng/ml (1:1000 dilution). At this dilution, we detected tubulin in extracts of Drosophila S2 cells, Xenopus A6 cells, mouse Swiss 3T3 cells, rat NRK cells, human HeLa cells, and bovine brain. For immunofluorescence, we used the anti-tubulin antibody to detect tubulin in mouse Swiss 3T3 cells. The antibody was used at a concentration of 2.5 μg/ml (1:200 dilution) followed by incubation with a 1:500 dilution of anti-sheep rhodamine-conjugated secondary antibody. This information is found usually in the figure legends or within the “Product Uses” section of our antibody datasheets.
FAQ from CYTOSKELETON
All dilutions are noted in the datasheet. The actual dilution of antibody depends on the cell/tissue type and experimental format being used. For western blotting, we have tested and recommend using the antibody at a concentration of 500 ng/ml (1:1000 dilution). At this dilution, we detected tubulin in extracts of Drosophila S2 cells, Xenopus A6 cells, mouse Swiss 3T3 cells, rat NRK cells, human HeLa cells, and bovine brain. For immunofluorescence, we used the anti-tubulin antibody to detect tubulin in mouse Swiss 3T3 cells. The antibody was used at a concentration of 2.5 μg/ml (1:200 dilution) followed by incubation with a 1:500 dilution of anti-sheep rhodamine-conjugated secondary antibody. This information is found usually in the figure legends or within the “Product Uses” section of our antibody datasheets.