Some Kits
Posted: Wed 29 Jun 2016 11:16Some kits
From CYTOSKELETON
Actin binding protein assay Biochem kit: This kit provides clear instructions and all the reagents and controls necessary to determine the ability of a given protein to bind to actin monomers versus actin filaments. F-actin binding can be measured by using a spin down assay where centrifugation is used to separate F-actin from G-actin by differential sedimentation. This kit can be used to determine whether a test protein binds F-actin or affects the equilibrium between G-actin and F-actin, whether a test protein has G-actin sequestering or F-actin polymerization enhancing activity or whether a test protein of interest can bundle F-actin. We suggest coupling this assay with the Actin polymerization assay Biochem kit in order to obtain a more complete profile of your potential actin binding protein.
Tubulin binding protein assay Biochem Kit: This kit provides clear instructions and all the reagents and controls necessary to determine the ability of a given protein to bind to tubulin monomers versus tubulin polymers (microtubules). Tubulin binding can be measured by using a spin down assay where centrifugation is used to separate microtubules from tubulin monomers by differential sedimentation. This kit can be used to: identify novel microtubule associated proteins (MAPs), confirm in vivo data suggesting a given protein is a MAP,characterize MAPs, identify/characterize MAP regulating proteins or identify/characterize compounds that inhibit MAP binding to microtubules. We suggest coupling this assay with the tubulin polymerization assay Biochem Kit in order to obtain a more complete profile of your potential tubulin binding protein.
Actin polymerization assay Biochem kit: This kit uses modified actin to follow polymerization kinetics. There are many types of actin binding proteins, including nucleating, capping, severing, side-binding and monomer sequestering actin binding proteins. All of these can be characterized by their effect on one or more of the three stages of actin polymerization: nucleation, growth and steady-state equilibrium. A compound’s effects on depolymerization can also be evaluated. Cytoskeleton has adapted this powerful assay into a user friendly Biochem kit format.
Tubulin polymerization assay Biochem kit: We offer both absorbance-based and fluorescence-based tubulin polymerization kits to follow polymerization kinetics. The absorbance-based kit takes advantage of the fact that light is scattered by microtubules to an extent that is proportional to the concentration of microtubule polymer. The fluorescence-based kit measures polymerization as a function of fluorescence enhancement following the incorporation of a fluorescent reporter into microtubules as polymerization occurs. There are many proteins and drugs that either enhance or inhibit polymerization or depolymerization. These compounds can be characterized by their effect on one or more of the three stages of tubulin polymerization: nucleation, growth, and steady-state equilibrium. A compound’s effects on depolymerization can also be evaluated. Cytoskeleton has adapted these powerful assays into user friendly Biochem Kit formats.
From CYTOSKELETON
Actin binding protein assay Biochem kit: This kit provides clear instructions and all the reagents and controls necessary to determine the ability of a given protein to bind to actin monomers versus actin filaments. F-actin binding can be measured by using a spin down assay where centrifugation is used to separate F-actin from G-actin by differential sedimentation. This kit can be used to determine whether a test protein binds F-actin or affects the equilibrium between G-actin and F-actin, whether a test protein has G-actin sequestering or F-actin polymerization enhancing activity or whether a test protein of interest can bundle F-actin. We suggest coupling this assay with the Actin polymerization assay Biochem kit in order to obtain a more complete profile of your potential actin binding protein.
Tubulin binding protein assay Biochem Kit: This kit provides clear instructions and all the reagents and controls necessary to determine the ability of a given protein to bind to tubulin monomers versus tubulin polymers (microtubules). Tubulin binding can be measured by using a spin down assay where centrifugation is used to separate microtubules from tubulin monomers by differential sedimentation. This kit can be used to: identify novel microtubule associated proteins (MAPs), confirm in vivo data suggesting a given protein is a MAP,characterize MAPs, identify/characterize MAP regulating proteins or identify/characterize compounds that inhibit MAP binding to microtubules. We suggest coupling this assay with the tubulin polymerization assay Biochem Kit in order to obtain a more complete profile of your potential tubulin binding protein.
Actin polymerization assay Biochem kit: This kit uses modified actin to follow polymerization kinetics. There are many types of actin binding proteins, including nucleating, capping, severing, side-binding and monomer sequestering actin binding proteins. All of these can be characterized by their effect on one or more of the three stages of actin polymerization: nucleation, growth and steady-state equilibrium. A compound’s effects on depolymerization can also be evaluated. Cytoskeleton has adapted this powerful assay into a user friendly Biochem kit format.
Tubulin polymerization assay Biochem kit: We offer both absorbance-based and fluorescence-based tubulin polymerization kits to follow polymerization kinetics. The absorbance-based kit takes advantage of the fact that light is scattered by microtubules to an extent that is proportional to the concentration of microtubule polymer. The fluorescence-based kit measures polymerization as a function of fluorescence enhancement following the incorporation of a fluorescent reporter into microtubules as polymerization occurs. There are many proteins and drugs that either enhance or inhibit polymerization or depolymerization. These compounds can be characterized by their effect on one or more of the three stages of tubulin polymerization: nucleation, growth, and steady-state equilibrium. A compound’s effects on depolymerization can also be evaluated. Cytoskeleton has adapted these powerful assays into user friendly Biochem Kit formats.