What kits to characterize a putative motor protein?
Posted: Wed 29 Jun 2016 11:41What kits are available to characterize a putative motor protein?
FAQ from CYTOSKELETON
Motor proteins utilize microtubules as a substrate, meaning that motor proteins are one type of microtubule-associated protein (MAP). To confirm that the putative motor protein binds with microtubules, Cytoskeleton’s microtubule binding protein spin down assay kit is available. The protein to be evaluated will need to be visualized by either Coomassie Blue or silver staining or with an antibody to either the protein itself or a tag conjugated to the protein (his, myc, etc.). A non-hydrolyzable ATP analog, e.g. AMPPNP, is usually used at 5 or 10 mM to maintain microtubule attachment. Microtubules also activate the ATPase activity of motor proteins which can be measured with one of our kinesin ATPase assays. These kits are useful for discovering kinesin inhibitors and activators as well as determining Vmax and Kcat values for a kinesin motor protein. Cytoskeleton, Inc. also offers a Biochem Kit which allows the visualization of motor protein motility using fluorescently-labeled microtubules.
FAQ from CYTOSKELETON
Motor proteins utilize microtubules as a substrate, meaning that motor proteins are one type of microtubule-associated protein (MAP). To confirm that the putative motor protein binds with microtubules, Cytoskeleton’s microtubule binding protein spin down assay kit is available. The protein to be evaluated will need to be visualized by either Coomassie Blue or silver staining or with an antibody to either the protein itself or a tag conjugated to the protein (his, myc, etc.). A non-hydrolyzable ATP analog, e.g. AMPPNP, is usually used at 5 or 10 mM to maintain microtubule attachment. Microtubules also activate the ATPase activity of motor proteins which can be measured with one of our kinesin ATPase assays. These kits are useful for discovering kinesin inhibitors and activators as well as determining Vmax and Kcat values for a kinesin motor protein. Cytoskeleton, Inc. also offers a Biochem Kit which allows the visualization of motor protein motility using fluorescently-labeled microtubules.