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Can Cytoskeleton offer custom purification of my protein?

PostPosted: Wed 29 Jun 2016 11:45
by Interchim
Can Cytoskeleton offer custom purification of my protein?
FAQ from CYTOSKELETON


Yes, Cytoskeleton, Inc. has an active contract services department that performs custom purifications to produce purified recombinant proteins with high biological activity. Examples of protein purification projects are:
Tubulin proteins isolated from cancer cell lines which are useful for developing drugs targeted toward cancer cells. We have isolated 1.0-5.0 mg of 90% pure tubulin from 50 g of cells.
Small G-protein Arf1 and Arf6 purified within 8 weeks to >85% purity and suitable for 10,000 GEF assays (50 x 1 mg).

We perform full quality control, which includes:
Full quantitation of yield, in mg per g tissue.
SDS-PAGE analysis of purified proteins, stained with Coomassie Blue and scanned with a densitometer to estimate purity.
Biological activity determination depending on the requirements of the customer.

The delivery time usually depends on the protein or type of assay, but 4 to 8 weeks is normal.