Staining F-actin with phalloidin
Posted: Wed 29 Jun 2016 11:32Staining F-actin with phalloidin
Technical Tip from CYTOSKELETON
When preparing cells for phalloidin staining, remember to fix cells in 4% paraformaldehyde rather than methanol. Methanol disrupts the structure of F-actin and the phalloidin binding site.
Technical Tip from CYTOSKELETON
When preparing cells for phalloidin staining, remember to fix cells in 4% paraformaldehyde rather than methanol. Methanol disrupts the structure of F-actin and the phalloidin binding site.