How avoid low recoveries of protein with new SW/SWXL column?
Posted: Wed 29 Jun 2016 12:28When I use a new SW/SWXL column I get low recoveries of my protein. How can I avoid this?
FAQ from TOSOH
There are active sites on the resin that require conditioning with either your sample protein or another material such as bovine serum albumin (BSA). For the SW/SWXL column we recommend 4 successive injections of 100 µg BSA to condition the resin. The PW/PWXL sizing columns are far less hydrophilic and normally do not require conditioning.
FAQ from TOSOH
There are active sites on the resin that require conditioning with either your sample protein or another material such as bovine serum albumin (BSA). For the SW/SWXL column we recommend 4 successive injections of 100 µg BSA to condition the resin. The PW/PWXL sizing columns are far less hydrophilic and normally do not require conditioning.