What factors consider when selecting the correct SEC column?
Posted: Wed 29 Jun 2016 13:34What factors should I consider when selecting the correct SEC column for my application?
FAQ from TOSOH
MW and molecular configuration, e.g. globular: Do they match to the fractionation range of the column.
Solvent solubility: what mobile phase is required and is the column compatible with this solvent, e.g. SW/SWXL column operate in the pH range of 2.5-7.5.
Hydrophilic vs. hydrophobic: this will dictate the ionic strength of the eluent used in the mobile phase. Some proteins have hydrophobic sites that require a small amount of organic solvent in the mobile phase such as 5% methanol. This inhibits secondary hydrophobic interaction.
Other factors: temperature, sample characteristics, HPLC system requirements, fittings required.
FAQ from TOSOH
MW and molecular configuration, e.g. globular: Do they match to the fractionation range of the column.
Solvent solubility: what mobile phase is required and is the column compatible with this solvent, e.g. SW/SWXL column operate in the pH range of 2.5-7.5.
Hydrophilic vs. hydrophobic: this will dictate the ionic strength of the eluent used in the mobile phase. Some proteins have hydrophobic sites that require a small amount of organic solvent in the mobile phase such as 5% methanol. This inhibits secondary hydrophobic interaction.
Other factors: temperature, sample characteristics, HPLC system requirements, fittings required.