How do I select an ion exchange resin?
Posted: Wed 29 Jun 2016 14:09How do I select an ion exchange resin?
FAQ from TOSOH
After identifying the isoelectric point of the compound of interest, select a mobile phase pH between the pI and the pKa of the ligand group. Elute the mixture from the column using a salt gradient, e.g. 0.3 mol/L NaCL to 1.0 mol/L NaCL.
FAQ from TOSOH
After identifying the isoelectric point of the compound of interest, select a mobile phase pH between the pI and the pKa of the ligand group. Elute the mixture from the column using a salt gradient, e.g. 0.3 mol/L NaCL to 1.0 mol/L NaCL.