Control elution volume in normal phase liquid chromatography
Posted: Wed 29 Jun 2016 14:34How do you control elution volume in normal phase liquid chromatography?
FAQ from TOSOH
Elution volume is controlled by the polarity of the mobile phase. By decreasing the mobile phase polarity (increasing the organic component) the sample is retained longer on the column. For example, oligosaccharides are very polar and require 40-50% water in the mobile phase in order to elute.
FAQ from TOSOH
Elution volume is controlled by the polarity of the mobile phase. By decreasing the mobile phase polarity (increasing the organic component) the sample is retained longer on the column. For example, oligosaccharides are very polar and require 40-50% water in the mobile phase in order to elute.