What factors affect the protein binding in HIC?
Posted: Thu 30 Jun 2016 09:19What factors affect the protein binding in HIC?
FAQ from TOSOH
A number of factors are involved in retention and elution of proteins by HIC, including the type of ligand and ligand density on the support resin, the hydrophobic nature of the protein, and the type and concentration of salt used. Thus, the analyst has several options when developing a HIC separation strategy.
FAQ from TOSOH
A number of factors are involved in retention and elution of proteins by HIC, including the type of ligand and ligand density on the support resin, the hydrophobic nature of the protein, and the type and concentration of salt used. Thus, the analyst has several options when developing a HIC separation strategy.