How do I select the optimal RP column for my application?
Posted: Thu 30 Jun 2016 09:29How do I select the optimal RP column for my application?
FAQ from TOSOH
The choice of an RPLC column is based upon the solubility and polarity of the test analytes. The more hydrophobic the sample molecule the less hydrophobic the solid support must be. Conversely, the less hydrophobic the sample molecule the more hydrophobic the solid support. For example, protein digests and low MW pharmaceuticals, waste water contaminants, etc. will generally be suited for a C18 column. More hydrophobic molecules with extensive aromatic nature may be best suited to C8 or phenyl ligands
FAQ from TOSOH
The choice of an RPLC column is based upon the solubility and polarity of the test analytes. The more hydrophobic the sample molecule the less hydrophobic the solid support must be. Conversely, the less hydrophobic the sample molecule the more hydrophobic the solid support. For example, protein digests and low MW pharmaceuticals, waste water contaminants, etc. will generally be suited for a C18 column. More hydrophobic molecules with extensive aromatic nature may be best suited to C8 or phenyl ligands