Fluorescently labeling a gene of interest & culture cells
Posted: Wed 8 Feb 2017 20:02For fluorescently labeling (tagging) a gene of interest, is it necessary to culture transfected cells to dilute cells containing donor DNA?
FAQ from OriGene
If in the donor construct the fluorescent protein does not have a mammalian expression promoter (for example the left homologous arm does not contain the promoter) , then you can sort the fluorescent cells out (you can tell in donor DNA only transfected cells); you might not need to get individual cell colonies. If the fluorescent protein in the donor template DNA does contain a mammalian expression promoter, you will need to pass the transfected cells several generations to dilute cells containing the donor construct.
Access to all Origenes' vectors
FAQ from OriGene
If in the donor construct the fluorescent protein does not have a mammalian expression promoter (for example the left homologous arm does not contain the promoter) , then you can sort the fluorescent cells out (you can tell in donor DNA only transfected cells); you might not need to get individual cell colonies. If the fluorescent protein in the donor template DNA does contain a mammalian expression promoter, you will need to pass the transfected cells several generations to dilute cells containing the donor construct.
Access to all Origenes' vectors