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Cas9 will not integrate in genome using lentivector ?

PostPosted: Wed 8 Feb 2017 20:09
by Interchim
How do you make sure that Cas9 will not integrate in genome if you use lentivector?
FAQ from OriGene

For screening purpose, for short term, integration of Cas9 into the genome for 2 weeks does not affect cells. You can use the integration-deficient lenti packaging kit to produce lentivirus that won’t integrate into the cellular genome, acting just like plasmid.

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