Transfect ES cells or pronuclei: for gene targeting in mice?
Posted: Wed 8 Feb 2017 20:13 For gene targeting in mice, do you recommend transfecting ES cells or pronuclei?
FAQ from OriGene
You can do both. You can inject mRNA (gRNA and Cas9 mRNA) or plasmid DNA (target sequence cloned pCas-Guide) into the zygotes or ES cells
Access to all Origenes' vectors
FAQ from OriGene
You can do both. You can inject mRNA (gRNA and Cas9 mRNA) or plasmid DNA (target sequence cloned pCas-Guide) into the zygotes or ES cells
Access to all Origenes' vectors