Using CRISPR, can you get monoallelic knockout or biallelic
Posted: Wed 8 Feb 2017 20:19Using CRISPR, can you get monoallelic knockout or biallelic knockout?
FAQ from OriGene
CRISPR/Cas9 double-strand cleavage is very efficient. If just using CRISPR/Cas9 vectors to introduce indels, if transfection efficiency is high, more biallelic knockout can occur. In the presence of donor DNA, since homologous recombination may be a limiting factor, some cells contain monoallelic knockout and some cells contain biallelic knock out.
Access to all Origenes' vectors
FAQ from OriGene
CRISPR/Cas9 double-strand cleavage is very efficient. If just using CRISPR/Cas9 vectors to introduce indels, if transfection efficiency is high, more biallelic knockout can occur. In the presence of donor DNA, since homologous recombination may be a limiting factor, some cells contain monoallelic knockout and some cells contain biallelic knock out.
Access to all Origenes' vectors