Screen edited cells after transfecting CRISPR/Cas9 vector
Posted: Wed 8 Feb 2017 20:29How to screen the edited cells after transfecting the CRISPR/Cas9 vector?
FAQ from OriGene
If it is gene knockout, you can do WB to verify it. You can also do genomic PCR to detect the genomic integration. For mutations, you can amplify the genomic sequence, and sequence it. If you do gene knockout, the selection marker in the donor template DNA will help the selection. If it is necessary, you can isolate individual cell colonies for introduction of specific mutations in the genome and other applications.
Access to all Origenes' vectors
FAQ from OriGene
If it is gene knockout, you can do WB to verify it. You can also do genomic PCR to detect the genomic integration. For mutations, you can amplify the genomic sequence, and sequence it. If you do gene knockout, the selection marker in the donor template DNA will help the selection. If it is necessary, you can isolate individual cell colonies for introduction of specific mutations in the genome and other applications.
Access to all Origenes' vectors