How to enrich CRISPR transfected cells?
Posted: Thu 9 Mar 2017 09:54The transfection efficiency of my cell line is only 20%, how to enrich CRISPR transfected cells?
FAQ from OriGene
You can use pCas-Guide-EF1a-GFP to sort out transfected GFP cells since GFP is also expressed. We also have a pCas-Guide-EF1a-CD4 vector, you can use CD4 antibody beads to enrich transfected cells.
Access to all Origenes' vectors
FAQ from OriGene
You can use pCas-Guide-EF1a-GFP to sort out transfected GFP cells since GFP is also expressed. We also have a pCas-Guide-EF1a-CD4 vector, you can use CD4 antibody beads to enrich transfected cells.
Access to all Origenes' vectors