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About the specific cleavage site of the Cas9:gRNA complex

PostPosted: Thu 9 Mar 2017 09:58
by Interchim
Do you know the specific cleavage site of the Cas9:gRNA complex in terms of where in the targeting sequence the cleaving occurs?
FAQ from OriGene

Cas9 cleaves at 3 bp away from the 3’ end of the target sequence in the genome.

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